Antifungal activity of three Chilean plant extracts on Botrytis cinerea
AbstractThe antifungal effect of the complete methanolic extract and a ethanolic fraction of three native Chilean plants (Ephedra breana, Fabiana imbricata, and Nolana sedifolia) were tested in vitro against Botrytis cinerea (the grey mold fungus) at 250 μg/mL for E. breana and N. sedifolia extracts and 400 μg/mL for F. imbricata extract. The results of this study showed that the ethanolic fractions of E. breana and N. sedifolia have a fungistatic effect during 14 days, while the fungus is exposed to the media with extracts. The complete methanolic fractions of the three studied plant species and the ethanolic fraction of F. imbricada did not show any fungicidal effect. The extracts and fractions were analyzed by high-performance liquid chromatography and the assayed compounds were: chlorogenic acid, cinnamic acid, p-coumaric acid, ferulic acid, vanillin, vanillic acid, rutin, caffeic acid, 3, 4-hydroxibenzoic acid (veratric acid), 3, 4-dimethoxycinnamic acid (caffeic acid dimethyl ester), and protocatechuic acid. Taking into account the antifungal activity of the ethanolic extracts of E. breana and N. sedifolia in in vitro assay, they may be an interesting alternative to control the phytopathogen Botrytis cinerea.
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