Comparison of the microbiome and mycobiome in tissues of the tropical carnivorous epiphytic herb Utricularia jamesoniana Oliv. (Lentibulariaceae)

keywords: bladderworts, carnivorous plants, bacteria, fungi, microbial diversity


Background: Utricularia jamesoniana, a small epiphytic plant found in wet tropical forests, stands out for its carnivorous habit, intricate trap system, and small but beautiful and complex flowers. This species remains relatively understudied despite its wide geographical distribution and curious adaptations.

Questions: Our aim is to elucidate the composition of bacterial and fungal communities inhabiting both the bladders and leaves of Utricularia jamesoniana, facilitating the comprehension of the physiology and ecological dynamics of this carnivorous species.

Methods: In this study, we employed 16S rRNA and ITS sequencing to compare the prokaryotic and fungal communities within leaves and traps of U. jamesoniana.

Results: The analysis of amplicon sequence variants (ASVs) unveiled notable differences in community composition depending on the plant tissue and type of microorganism. Prokaryotic communities predominantly comprised Proteobacteria and Actinobacteriota, featuring genera such as Acidocella, Bradyrhizobium, Ferritrophicum, and Ferrovum. Fungal communities were dominated by Ascomycota and Basidiomycota, encompassing representatives of Dothideomycetes, Sordariomycetes, Eurotiomycetes, and Agaricomycetes, as well as ASVs related to Mycosphaerellaceae, Colletotrichum, Aspergillus, and Thanatephorus. We determined that the prokaryotic diversity was higher in the bladders with respect to the leaves. Fungal communities, in turn, were more diverse in leaves than in bladders.

Conclusions: This study sheds light on the microbial communities associated with this carnivorous epiphyte and provides valuable insights into the intricate relationships between the plant and its microbial inhabitants across different tissues.


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Comparison of the microbiome and mycobiome in tissues of the tropical carnivorous epiphytic herb <em>Utricularia jamesoniana</em> Oliv. (Lentibulariaceae)


Adamec L. 2007. Oxygen concentrations inside the traps of the carnivorous plants Utricularia and Genlisea (Lentibulariaceae). Annals of Botany 100: 849-856. DOI:

Adamec L. 2011. Functional characteristics of traps of aquatic carnivorous Utricularia species. Aquatic Botany 95: 226-233. DOI:

Adamec L, Sirová D, Vrba J, Rejmánková E. 2010. Enzyme production in the traps of aquatic Utricularia species. Biologia 65: 273-278. DOI:

Afzal I, Shinwari ZK, Sikandar S, Shahzad S. 2019. Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research 221: 36-49. DOI:

Alcaraz LD, Martínez-Sánchez S, Torres I, Ibarra-Laclette E, Herrera-Estrella L. 2016. The metagenome of Utricularia gibba’s traps: Into the microbial input to a carnivorous plant. Wilson BA. ed. Plos One 11: e0148979. DOI:

Alkhalaf IA, Hübener T, Porembski S. 2009. Prey spectra of aquatic Utricularia species (Lentibulariaceae) in northeastern Germany: The role of planktonic algae. Flora - Morphology, Distribution, Functional Ecology of Plants 204: 700-708. DOI:

Anderson NA. 1982. The genetics and pathology of Rhizoctonia solani. Annual Review of Phytopathology 20: 329-347. DOI:

Behie SW, Jones SJ, Bidochka MJ. 2015. Plant tissue localization of the endophytic insect pathogenic fungi Metarhizium and Beauveria. Fungal Ecology 13: 112-119. DOI:

Brader G, Compant S, Vescio K, Mitter B, Trognitz F, Ma L-J, Sessitsch A. 2017. Ecology and genomic insights into plant-pathogenic and plant-nonpathogenic endophytes. Annual Review of Phytopathology 55: 61-83. DOI:

Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. 2016. DADA2: High-resolution sample inference from Illumina amplicon data. Nature Methods 13: 581-583. DOI:

Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Lozupone CA, Turnbaugh PJ, Fierer N, Knight R. 2011. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proceedings of the National Academy of Sciences of the United States of America 108: 4516-4522. DOI:

Caravieri FA, Ferreira AJ, Ferreira A, Clivati D, de Miranda VFO, Araújo WL. 2014. Bacterial community associated with traps of the carnivorous plants Utricularia hydrocarpa and Genlisea filiformis. Aquatic Botany 116: 8-12. DOI:

Chan XY. 2019. Microbial diversity and bacterial biocatalytic activities in pitcher fluid of Nepenthes sp. Ph.D. Thesis. Universiti Malaya

Crow GE. 2007. Lentibulariaceae. In: Hammel BE, Grayum MH, Herrera C, and Zamora N. eds. Manual de Plantas de Costa Rica, Volumen VI, Dicotiledóneas (Haloragaceae-Phytolaccaceae), Monographs in Systematic Botany from the Missouri Botanical Garden, Missouri. pp. 189-197. ISBN: 978-1930723603.

Deban SM, Holzman R, Müller UK. 2020. Suction feeding by small organisms: performance limits in larval vertebrates and carnivorous plants. Integrative and Comparative Biology 60: 852-863. DOI:

Deshpande V, Wang Q, Greenfield P, Charleston M, Porras-Alfaro A, Kuske CR, Cole JR, Midgley DJ, Tran-Dinh N. 2015. Fungal identification using a Bayesian classifier and the Warcup training set of internal transcribed spacer sequences. Mycologia 108:14-293. DOI:

Donk MA. 1956. Notes on resupinate Hymenomycetes-II* The tulasnelloid fungi. Reinwardtia 3: 363-379.

Ellison A, Adamec L. 2018. Carnivorous plants: physiology, ecology, and evolution. United Kingdom Oxford: Oxford University Press, ISBN: 978-0198779841

Fleischmann A. 2015. Taxonomic Utricularia news. Carnivorous Plant Newsletter 44: 13-16. DOI:

Friday LE. 1989. Rapid turnover of traps in Utricularia vulgaris L. Oecologia 80: 272-277. DOI:

Givnish TJ, Sparks KW, Hunter SJ, Pavlovič A. 2018. Why are plants carnivorous? Cost/benefit analysis, whole-plant growth, and the context-specific advantages of botanical carnivory. Oxford, United Kingdom: Oxford University Press. DOI:

Glenn A, Bodri MS. 2012. Fungal endophyte diversity in Sarracenia. Plos One 7: e32980. DOI:

Gomes Rodrigues F, Franco Marulanda N, Silva SR, Płachno BJ, Adamec L, Miranda VFO. 2017. Phylogeny of the ‘orchid-like’ bladderworts (gen. Utricularia sect. Orchidioides and Iperua: Lentibulariaceae) with remarks on the stolon-tuber system. Annals of Botany 120: 709-723. DOI:

Grothjan JJ, Young EB. 2022. Bacterial recruitment to carnivorous pitcher plant communities: identifying sources influencing plant microbiome composition and function. Frontiers in Microbiology 13: 791079. DOI:

Guedes FM, Gonella PM, Domínguez Y, Moreira ADR, Silva SR, Díaz YCA, Fleischmann A. Menezes CG, Rivadavia F, Miranda VFO. 2023. Utricularia in Flora e Funga do Brasil. Jardim Botânico do Rio de Janeiro.

Hardoim PR, van Overbeek LS, Berg G, Pirttilä AM, Compant S, Campisano A, Döring M, Sessitsch A. 2015. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews 79: 293-320. DOI:

Henning T, Allen JP, Rodríguez Rodríguez EF. 2021. A new species of Utricularia Sect. Orchidioides (Lentibulariaceae) from the Amotape-Huancabamba Zone of North Peru. Darwiniana, nueva serie 9: 299-311. DOI:

Hung R, Rutgers LS. 2016. Applications of Aspergillus in Plant Growth Promotion. In: Vijai Kumar Gupta ed. New and Future Developments in Microbial Biotechnology and Bioengineering. Tallinn, Estonia: Elsevier, pp. 223-227. DOI:

Ibarra-Laclette E, Lyons E, Hernández-Guzmán G, Pérez-Torres CA, Carretero-Paulet L, Chang T-H, Lan T, Welch AJ, Juárez MJA, Simpson J, Fernández-Cortés A, Arteaga-Vázquez M, Góngora-Castillo E, Acevedo-Hernández G, Schuster SC, Himmelbauer H, Minoche AE, Xu S, Lynch M, Oropeza-Aburto A, Cervantes-Pérez SA, de Jesús Ortega-Estrada M, Cervantes-Luevano JI, Michael TP, Mockler T, Bryant D, Herrera-Estrella A, Albert VA, Herrera-Estrella L. 2013. Architecture and evolution of a minute plant genome. Nature 498: 94-98. DOI:

Jobson RW, Baleeiro PC, Guisande C. 2018. Systematics and evolution of Lentibulariaceae: III. Utricularia. In: Ellison A, Adamec L, eds. Carnivorous Plants: Physiology, Ecology, and Evolution. Oxford, United Kingdom: Oxford University Press, pp.89-104. DOI:

Jobson RW, Morris EC. 2001. Feeding ecology of a carnivorous bladderwort (Utricularia uliginosa, Lentibulariaceae). Austral Ecology 26: 680-691. DOI:

Johnson DB, Hallberg KB, Hedrich S. 2014. Uncovering a microbial enigma: Isolation and characterization of the streamer-generating, iron-oxidizing, acidophilic bacterium “Ferrovum myxofaciens.” Applied and Environmental Microbiology 80: 672-680. DOI:

Lee J, Tan W, Ting A. 2014. Revealing the antimicrobial and enzymatic potentials of culturable fungal endophytes from tropical pitcher plants (Nepenthes spp.). Mycosphere 5: 364-377. DOI:

Martens A, Grabow K. 2011. Early stadium damselfly larvae (Odonata: Coenagrionidae) as prey of an aquatic plant, Utricularia australis. International Journal of Odonatology 14: 101-104. DOI:

Miranda VFO, Silva SR, Reut MS, Dolsan H, Stolarczyk P, Rutishauser R, Plachno BJ. 2021. A Historical perspective of bladderworts (Utricularia): Traps, carnivory and body architecture. Plants 10: 2656. DOI:

Moseley HN. 1884. The fish-eating Utricularia, or bladderwort. Bulletin of the United States Fish Commission. Plate II:261. Murali A, Bhargava A, Wright ES. 2018. IDTAXA: a novel approach for accurate taxonomic classification of microbiome sequences. Microbiome 6: 140. DOI:

Naseem F, Kayang H. 2018. Fungal endophytes associated with Nepenthes khasiana Hook. f., an endemic plant of meghalaya, India. International Journal of Current Research in Life Sciences 7: 1907-1912.

Okamoto R, Kojima H, Fukui M. 2017. Acidocella aquatica sp. nov., a novel acidophilic heterotrophic bacterium isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 67: 4773-4776. DOI:

Oksanen J, Blanchet FG, Friendly M, Kindt R, Legendre P, McGlinn D, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens MHH, Szoecs E, Wagner H. 2022. vegan: Community Ecology Package. R package.

Oliver D. 1860. Descriptions of new species of Utricularia from South America, with notes upon, the genera Polypompholyx and Akentra. Botanical Journal of the Linnean Society 4: 169-176. DOI:

Ortega Ardila AT, Romero Salgado JO. 2016. Plantas carnívoras de Virolín (Santander, Colombia): una guía de campo. Bsc Thesis. Universidad Pedagógica Nacional.

Peroutka M, Adlassnig W, Volgger M, Lendl T, Url WG, Lichtscheidl IK. 2008. Utricularia: a vegetarian carnivorous plant? Plant Ecology 199: 153-162. DOI:

Płachno BJ, Adamec L, Lichtscheidl IK, Peroutka M, Adlassnig W, Vrba J. 2006. Fluorescence labelling of phosphatase activity in digestive glands of carnivorous plants. Plant Biology 8: 813-820. DOI:

Płachno BJ, Łukaszek M, Wołowski K, Adamec L, Stolarczyk P. 2012. Aging of Utricularia traps and variability of microorganisms associated with that microhabitat. Aquatic Botany 97: 44-48. DOI:

Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research 41: D590-D596. DOI:

Quilliam RS, Jones DL. 2010. Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 20: 341-348. DOI:

R Core Team. 2023. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. URL

Richards JH. 2001. Bladder function in Utricularia purpurea (Lentibulariaceae): is carnivory important? American Journal of Botany 88: 170-176. DOI:

Rueda-Almazán JE, Hernández VM, Alcalá-Martínez JR, Fernández-Duque A, Ruiz-Aguilar M, Alcalá RE. 2021. Spatial and temporal differences in the community structure of endophytic fungi in the carnivorous plant Pinguicula moranensis (Lentibulariaceae). Fungal Ecology 53: 101087. DOI:

Rutishauser R. 2016. Evolution of unusual morphologies in Lentibulariaceae (bladderworts and allies) and Podostemaceae (river-weeds): a pictorial report at the interface of developmental biology and morphological diversification. Annals of Botany 117: 811-832. DOI:

Sanabria-Aranda L, González-Bermudez A, Torres N, Ned Guisande C, Manjarres-Hernandez A, Valoyes-Valois V, Diaz-Olarte J, Andrade-Sossa C, Duque SR. 2006. Predation by the tropical plant Utricularia foliosa. Freshwater Biology 51: 1999-2008. DOI:

Sickel W, Van de Weyer A-L, Bemm F, Schultz J, Keller A. 2019. Venus flytrap microbiotas withstand harsh conditions during prey digestion. FEMS Microbiology Ecology 95: 10. DOI:

Silva SR, Gibson R, Adamec L, Domínguez Y, Miranda VFO. 2018. Molecular phylogeny of bladderworts: A wide approach of Utricularia (Lentibulariaceae) species relationships based on six plastidial and nuclear DNA sequences. Molecular Phylogenetics and Evolution 118: 244-264. DOI:

Singh K, Reyes RC, Campa G, Brown MD, Hidalgo F, Berg O, Müller UK. 2020. Suction flows generated by the carnivorous bladderwort Utricularia-comparing experiments with mechanical and mathematical Mmodels. Fluids 5: 33. DOI:

Sirová D, Adamec L, Vrba J. 2003. Enzymatic activities in traps of four aquatic species of the carnivorous genus Utricularia. New Phytologist 159: 669-675. DOI:

Sirová D, Bárta J, Borovec J, Vrba J. 2018a. The Utricularia-associated microbiome: composition, function, and ecology. In: Ellison A, Adamec L, eds. Carnivorous Plants: Physiology, Ecology, and Evolution. Oxford, United Kingdom: Oxford University Press, pp. 349-358. DOI:

Sirová D, Bárta J, Šimek K, Posch T, Pech J, Stone J, Borovec J, Adamec L, Vrba J. 2018b. Hunters or farmers? Microbiome characteristics help elucidate the diet composition in an aquatic carnivorous plant. Microbiome 6: 225. DOI:

Sirová D, Borovec J, Černá B, Rejmánková E, Adamec L, Vrba J. 2009. Microbial community development in the traps of aquatic Utricularia species. Aquatic Botany 90: 129-136. DOI:

Taylor P. 1989. The genus Utricularia. A taxonomic monograph. United Kingdom: Royal Botanic Gardens, Kew, 724 p. ISBN: 9780947643720.

Tosi J.A. 1969. Ecological map of the life zones of Costa Rica. Tropical Science Center, San Jose, Costa Rica. Map (1:1.000.000 scale).

Valdés CMP. 2008. El género Utricularia (lentibulariaceae) en Las Antillas Mayores. Revista del Jardín Botánico Nacional 29: 11-20.

VanInsberghe D, Maas KR, Cardenas E, Strachan CR, Hallam SJ, Mohn WW. 2015. Non-symbiotic Bradyrhizobium ecotypes dominate North American forest soils. The ISME Journal 9: 2435-2441. DOI:

Walker I. 2004. Trophic interactions within the Utricularia habitat in the reservoir of the Balbina hydroelectric powerplant (Amazonas, Brazil). Acta Limnologica Brasiliensia 16: 183-191.

Wang Q, Garrity GM, Tiedje JM, Cole JR. 2007. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73: 5261-5267. DOI:

Weiss J V, Rentz JA, Plaia T, Neubauer SC, Merrill-Floyd M, Lilburn T, Bradburne C, Megonigal JP, Emerson D. 2007. Characterization of neutrophilic Fe(II)-oxidizing bacteria isolated from the rhizosphere of wetland plants and description of Ferritrophicum radicicola gen. nov. sp. nov., and Sideroxydans paludicola sp. nov. Geomicrobiology Journal 24: 559-570. DOI:

Westermeier AS, Fleischmann A, Müller K, Schäferhoff B, Rubach C, Speck T, Poppinga S. 2017. Trap diversity and character evolution in carnivorous bladderworts (Utricularia, Lentibulariaceae). Scientific Reports 7: 12052. DOI:

Wu L, Han T, Li W, Jia M, Xue L, Rahman K, Qin L. 2013. Geographic and tissue influences on endophytic fungal communities of Taxus chinensis var. mairei in China. Current Microbiology 66: 40-48. DOI:

Zahran HH. 1999. Rhizobium -legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiology and Molecular Biology Reviews 63: 968-989. DOI:

How to Cite
Naranjo-Aguilar, V., Mora-Castro, R., Morera-Huertas, J., Acuña-Castillo, R. H., & Rojas-Jimenez, K. (2024). Comparison of the microbiome and mycobiome in tissues of the tropical carnivorous epiphytic herb Utricularia jamesoniana Oliv. (Lentibulariaceae). Botanical Sciences, 102(2), 401-415.