Evolutionary approaches in ethnobotany of medicinal plants and bioprospecting

  • Madeleyne Cupido Programa Multidisciplinario de Posgrado en Ciencias Ambientales, Universidad Autónoma de San Luis Potosí, San Luis Potosí https://orcid.org/0000-0002-5017-3992
  • José Arturo De-Nova Instituto de Investigación de Zonas Desérticas, Universidad Autónoma de San Luis Potosí, San Luis Potosí https://orcid.org/0000-0002-1989-1355
  • Virginia Gabriela Cilia-López Facultad de Medicina-Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología de la Universidad Autónoma de San Luis Potosí, San Luis Potosí https://orcid.org/0000-0003-0064-4490
keywords: Biodiversity, evosystem, phylogeny


Evolution has originated high levels of biodiversity that provide essential benefits to humans called option values of biodiversity. These resources are extracted and used to meet basic needs, such as traditional medicine, which is the result of human experience and its contact with nature over time. Traditional knowledge used as a guide for bioprospecting and ethnomedicinal research is the first step to protecting it and making it visible. The need to apply phylogenetic approaches and their importance for the development of ethnobotany in Mexico is discussed, as well as their implications for the discovery, use and conservation of medicinal resources for human well-being. This approach, elucidating the evolutionary history of species, can improve bioprospecting practices since they point out related species which could produce useful chemical compounds. This new approach is relevant to biotechnology and emphasizes the importance of preserving biocultural heritage.


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Author Biography

José Arturo De-Nova, Instituto de Investigación de Zonas Desérticas, Universidad Autónoma de San Luis Potosí, San Luis Potosí

My research focuses on the application of molecular tools to basic questions of plant phylogeny (evolutionary relationships), biogeography, and diversity. Major topics of current investigation include phylogenetic  diversity studies of the Mexican flora.

Evolutionary approaches in ethnobotany of medicinal plants and bioprospecting


Albuquerque UP, Medeiros PM. 2013. What is evolutionary ethnobiology? Ethnobiology and Conservation 2: 6. https://doi.org/10.15451/ec2013-8-2.6-1-04

Amiguet VT, Arnason JT, Maquin P, Cal V, Sanchez-Vindas P, Poveda-Alvarez. 2006. A regression analysis of q‘eqchi’ Maya medicinal plants from southern Belize. Economic Botany 60: 24-38. DOI: https://doi.org/10.1663/0013-0001(2006)60[24:ARAOQM]2.0.CO;2

Amrita M, Arun KM. 2019. Development of herbal tablet formulation: Systematic approach. Alternative and Integrative Medicine 8: 1.

Ankli A, Sticher O, Heinrich M. 1999a. Yucatec Maya medicinal plants versus nonmedicinal plants: indigenous characterization and selection. Human Ecology 27: 557-580. DOI: https://doi.org/10.1023/A:1018791927215

Ankli A, Sticher O, Heinrich M. 1999b. Medical ethnobotany of the Yucatec Maya: healers' consensus as a quantitative criterion. Economic Botany 53: 144-160. DOI: https://doi.org/10.1007/BF02866493

Bennett BC, Husby CE. 2008. Patterns of medicinal plant use: An examination of the Ecuadorian Shuar medicinal flora using contingency table and binomial analyses. Journal of Ethnopharmacology 116: 422-430. DOI: https://doi.org/10.1016/j.jep.2007.12.006

Blanckaert I, Paredes-Flores M, Espinosa-García FJ, Pinero D, Lira R. 2012. Ethnobotanical, morphological, phytochemical and molecular evidence for the incipient domestication of Epazote (Chenopodium ambrosioides L.: Chenopodiaceae) in a semi-arid region of Mexico. Genetic Resources and Crop Evolution 59: 557-573. DOI: https://doi.org/10.1007/s10722-011-9704-7

Bletter N. 2007. A quantitative synthesis of the medicinal ethnobotany of the Malinké of Mali and the Asháninka of Peru, with a new theoretical framework. Journal Ethnobiology and Ethnomedicine 3:36. https://doi.org/10.1186/1746-4269-3-36

Borchardt JK. 2002. The Beginnings of drug therapy: ancient mesopotamian medicine. Drug News & Perspectives 15: 187-192. DOI: https://doi.org/10.1358/dnp.2002.15.3.840015

Casagrande DG. 2000. Human taste and cognition in Tzeltal Maya medicinal plant use. Journal of Ecological Anthropology 4: 57-69. DOI: https://doi.org/10.5038/2162-4593.4.1.3

CBD. 2010. Convenio sobre la Diversidad Biológica. https://www.cbd.int/undb/media/factsheets/undb-factsheets-es-web.pdf (Accessed January 11, 2023).

CBD. 2017. Primer Informe Nacional Provisional sobre la Implementación del Protocolo de Nagoya en México. https://absch.cbd.int/es/database/NR/ABSCH-NR-MX-238713/1 (Accessed September 23, 2023).

Chen S, Li Q, McClements DJ, Han Y, Dai L, Mao L, Gao Y. 2020. Co-delivery of curcumin and piperine in zein-carrageenan core-shell nanoparticles: Formation, structure, stability and in vitro gastrointestinal digestion. Food Hydrocolloids 99: 105334. DOI: https://doi.org/10.1016/j.foodhyd.2019.105334

Cragg GM, Newman DJ. 2013. Natural products: a continuing source of novel drug leads. Biochimica et Biophysica Acta (BBA)-General Subjects 1830: 3670-3695. DOI: https://doi.org/10.1016/j.bbagen.2013.02.008

Cupido M, De-Nova A, Guerrero-González ML, Pérez-Vázquez FJ, Méndez-Rodríguez KB, Delgado-Sánchez P. 2022. GC-MS analysis of phytochemical compounds of Opuntia megarrhiza (Cactaceae), an endangered plant of Mexico. PeerJ Organic Chemistry 4: e5. DOI: https://doi.org/10.7717/peerj-ochem.5

Cupido M, De-Nova JA, Cilia-López G. 2023. Catálogo de plantas medicinales de la región biocultural Huasteca. Proyecto CONACYT Ciencia de Frontera 320270. Universidad Autónoma de San Luis Potosí, México. https://biocultural.uaslp.mx (Accessed March 1, 2023)

Dalton R. 2004. Natural resources: bioprospects less than golden. Nature 429: 598-600. DOI: https://doi.org/10.1038/429598a

De-Nova JA, Villegas-Ortega DS, Cupido M, Cilia-López VG. Evolutionary clustering in neotropical biocultural heritage: the Huastec Mayan useful plants. Botanical Journal of the Linnean Society (aceptado). DOI: https://doi.org/10.1093/botlinnean/boad061

Díaz-Toribio MH, De-Nova JA, Piedra-Malagón EM, Angulo DF, Sosa V. 2023. Wild and cultivated comestible plant species in the Gulf of Mexico: phylogenetic patterns and convergence of type of use. AoB Plants 15: 1-12. DOI: https://doi.org/10.1093/aobpla/plad063

DOF [Diario Oficial de la Federación]. 2014. Decreto Promulgatorio del Protocolo de Nagoya sobre Acceso a los Recursos Genéticos y Participación Justa y Equitativa en los Beneficios que se Deriven de su Utilización al Convenio sobre la Diversidad Biológica, adoptado en Nagoya el veintinueve de octubre de dos mil diez. México D.F. https://www.dof.gob.mx/nota_detalle.php?codigo=5363605&fecha=10/10/2014#gsc.tab=0 (Accessed 4 Sep, 2023).

Douwes E, Crouch NR, Edwards TJ, Mulholland DA. 2008. Regression analyses of southern African ethnomedicinal plants: informing the targeted selection of bioprospecting and pharmacological screening subjects. Journal of Ethnopharmacology 119: 356-364. DOI: https://doi.org/10.1016/j.jep.2008.07.040

Faith DP, Magallón S, Hendry AP, Conti E, Yahara T, Donoghue MJ. 2010. Evosystem services: an evolutionary perspective on the links between biodiversity and human well-being. Current Opinion in Environmental Sustainability 2: 66-74. DOI: https://doi.org/10.1016/j.cosust.2010.04.002

Ferreira-Júnior WS, Medeiros PM, Albuquerque PU. 2022. Evolutionary ethnobiology. Ethnobiology & Conservation 11: 1-8. DOI: https://doi.org/10.15451/ec2022-04-11.10-1-8

Flores-Camargo FG, Sánchez-Dirzo MG. 2022. Maximino: Base de datos de plantas útiles de México. https://www.maximinom.org (Accessed July 1, 2023)

Futuyma DJ. 1995. The uses of evolutionary biology. Science 267: 41-42. DOI: https://doi.org/10.1126/science.7809608

Galm U, Shen B. 2007. Natural product drug discovery: The times have never been better. Chemistry & Biology 14: 1098-1104. DOI: https://doi.org/10.1016/j.chembiol.2007.10.004

Garnatje T, Peñuelas J, Vallès J. 2017. Ethnobotany, Phylogeny, and ‘Omics’ for Human Health and Food Security. Trends in Plant Science 22: 187-191. DOI: https://doi.org/10.1016/j.tplants.2017.01.001

Geck MS, Cristians S, Berger-González M, Casu L, Heinrich M, Leonti M. 2020. Traditional Herbal Medicine in Mesoamerica: Toward Its Evidence Base for Improving Universal Health Coverage. Frontiers in Pharmacology 11: 1-49. DOI: https://doi.org/10.3389/fphar.2020.01160

Hamilton GR, Baskett TF. 2000. In the arms of Morpheus the development of morphine for postoperative pain relief. Canadian Journal of Anaesthesia 47: 367-374. DOI: https://doi.org/10.1007/BF03020955

Hao DC, Xiao PG. 2015. Genomics and Evolution in Traditional Medicinal Plants: Road to a Healthier Life. Evolutionary Bioinformatics 11: 197-212. DOI: https://doi.org/10.4137/EBO.S31326

Hart KH, Cox PA. 2000. A cladistic approach to comparative ethnobotany: Dye plants of the the southwestern United States. Journal of Ethnobiology 20: 303-325.

Heinrich M, Ankli A, Frei B, Weimann C, Sticher O. 1998. Medicinal plants in Mexico: healers' consensus and cultural importance. Social Science & Medicine 47: 1859-1871. DOI: https://doi.org/10.1016/s0277-9536(98)00181-6

Hernández‐Hernández T, Brown JW, Schlumpberger BO, Eguiarte LE, Magallón S. 2014. Beyond aridification: multiple explanations for the elevated diversification of cacti in the New World Succulent Biome. New phytologist 202: 1382-1397. DOI: https://doi.org/10.1111/nph.12752

Hernández-Ordoñez SR. 2019. El protocolo de Nagoya en México: un análisis legal del cumplimiento y el papel de los protocolos comunitarios bioculturales. Revista de la Facultad de Derecho de México 69: 611-646. DOI: http://dx.doi.org/10.22201/fder.24488933e.2019.275-2.69422

Joo YE. 2014. Natural product-derived drugs for the treatment of inflammatory bowel diseases. Intestinal Research 12: 103-109. DOI: https://doi.org/10.5217/ir.2014.12.2.103

Kumadoh D, Kwakye KO. 2017. Dosage forms of herbal medicinal products and their stability considerations-an overview. Journal of Critical Reviews 4: 1-8.

Leonti M. 2011. The future is written impact of scripts on the cognition, selection, knowledge and transmission of medicinal plant use and its implications for ethnobotany and ethnopharmacology. Journal of Ethnopharmacology 134: 542-555. DOI: https://doi.org/10.1016/j.jep.2011.01.017

Mawalagedera SM, Symonds MR, Callahan DL, Gaskett AC, Rønsted N. 2019. Combining evolutionary inference and metabolomics to identify plants with medicinal potential. Frontiers in Ecology and Evolution 7: 267. DOI: https://doi.org/10.3389/fevo.2019.00267

Melgar-Fernández M. 2005. Biotecnología y propiedad intelectual: un enfoque integrado desde el derecho internacional. DF, México: Universidad Nacional Autónoma de México. ISBN 970-32-2552-7

Medeiros PM, Albuquerque UP. 2015. Use Patterns of Medicinal Plants by Local Populations. In: Alburquerque UP, Madeiros PM, Casas A, eds. Evolutionary Ethnobiology. Switzerland: Springer, pp. 7-20. DOI: https://doi.org/10.1007/978-3-319-19917-7

Moerman DE. 1991. The medicinal flora of native North-America - an analysis. Journal of Ethnopharmacology 31: 1-42. DOI: http://dx.doi.org/10.1016/0378-8741(91)90141-Y

Moerman DE, Pemberton RW, Kiefer D, Berlin B. 1999. A comparative analysis of five medicinal floras. Journal of Ethnobiology 1: 49-67.

Molina-Venegas R, Fischer M, Mollel NP, Hemp A. 2020. Connecting plant evolutionary history and human well-being at Mt. Kilimanjaro, Tanzania. Botanical Journal of the Linnean Society 194: 397–409. DOI: https://doi.org/10.1093/botlinnean/boaa049

Molina-Venegas R. 2021. Conserving evolutionarily distinct species is critical to safeguard human well‐being. Scientific Reports 11: 24187. https://doi.org/10.1038/s41598-021-03616-x

Muñetón-Pérez P. 2009. Plantas medicinales: un comPlemento vital Para la salud de los mexicanos. entrevista con el Dr. Erick Estrada Lugo. Revista Digital Universitaria 10: 1-9.

Mushtaq S, Abbasi BH, Uzair B, Abbasi R. 2018. Natural products as reservoirs of novel therapeutic agents. Journal Experimental and Clinical Sciences 17: 420. DOI: https://doi.org/10.17179/excli2018-1174

Newman DJ, Cragg GM, Snader KM. 2003. Natural products as sources of new drugs over the period 1981-2002. Journal of Natural Products 7: 1022-1037. DOI: https://doi.org/10.1021/np030096l

Patwardhan B. 2005. Ethnopharmacology and drug discovery. Journal of Ethnopharmacology 100: 50-52. DOI: https://doi.org/10.1016/j.jep.2005.06.006

Poulose N, Sajayan A, Ravindran A, Sreechithra TV, Vardhan V, Selvin J, Kiran GS. 2020. Photoprotective effect of nanomelanin-seaweed concentrate in formulated cosmetic cream: With improved antioxidant and wound healing properties. Journal of Photochemistry and Photobiology B: Biology 205: 111816. DOI: https://doi.org/10.1016/j.jphotobiol.2020.111816

Prance GT, Chadwick D, Marsh J. 1994. Ethnobotany and the Search for New Drugs. Chichester, UK: John Wiley & Sons. ISBN: 0471950246

Reid WV, Laird S, Meyer CA, Gámez R, Sittenfeld A, Janzen DH, Gollin MA, Juma C. 1993. Biodiversity Prospecting: Using Genetic Resources for Sustainable Development. Washington, DC: World Resources Institute. ISBN: 0-915825-89-9

Rønsted N, Savolainen V, Mølgaard P, Jäger AK. 2008. Phylogenetic selection of Narcissus species for drug discovery. Biochemical Systematics and Ecology 36: 417-422. DOI: https://doi.org/10.1016/j.bse.2007.12.010

Rønsted N, Symonds MRE, Birkholm T, Birkholm T, Brøgger S, Meerow AW, Molander M, Mølgaard P, Petersen G, Rasmussen N, van Staden J, Stafford GI, Jäger AK. 2012. Can phylogeny predict chemical diversity and potential medicinal activity of plants? A case study of amaryllidaceae. BMC Ecology and Evolution 12: 182 DOI: https://doi.org/10.1186/1471-2148-12-182

Salazar-Aranda R, Pérez-López LA, Rivas-Galindo V, de Torres NW. 2013. Antimicrobial activity of plants used in México for gastrointestinal and respiratory disorders. In: Shahid M, Malik A, ASahai S. eds. Recent Trends in Biotechnology and Therapeutic Applications of Medicinal Plants. New York London: Dordrecht Springer. pp. 131-188. ISBN: 978-94-007-6602-0 DOI: https://doi.org/10.1007/978-94-007-6603-7_7

Sansores-España D, Pech-Aguilar AG, Cua-Pech KG, Medina-Vera I, Guevara-Cruz M, Gutiérrez-Solis AL, Reyes-García JG, Avila-Nava A. 2022. Plants Used in Mexican Traditional Medicine for the Management of Urolithiasis: A Review of Preclinical Evidence, Bioactive Compounds, and Molecular Mechanisms. Molecules 27: 2008. https://doi.org/10.3390/molecules27062008

Santoro FR, Nascimento ALB, Soldati GT, Ferreira Júnior WS, Albuquerque UP. 2018. Evolutionary ethnobiology and cultural evolution: opportunities for research and dialog. Journal of Ethnobiology and Ethnomedicine 14: 1-14. DOI: https://doi.org/10.1186/s13002-017-0199-y

Saslis-Lagoudakis CH, Williamson EM, Savolainen V, Hawkins JA. 2011a. Cross-cultural comparison of three medicinal floras and implications for bioprospecting strategies. Journal of Ethnopharmacology 135: 476-487. DOI: https://doi.org/10.1016/j.jep.2011.03.044

Saslis-Lagoudakis CH, Klitgaard BB, Forest F, Francis L, Savolainen V, Williamson EM, Hawkins JA. 2011b. The use of phylogeny to interpret cross-cultural patterns in plant use and guide medicinal plant discovery: an example from Pterocarpus (Leguminosae). Plos One 6: e22275. DOI: https://doi.org/10.1371/journal.pone.0022275

Saslis-Lagoudakis CH, Savolainen V, Williamson EM, Forest F, Wagstaff SJ, Baral SR, Watson FM, Colin AP, Hawkins JA. 2012. Phylogenies reveal predictive power of traditional medicine in bioprospecting. Proceedings of the National Academy of Sciences 39: 15835-15840. DOI: https://doi.org/10.1073/pnas.1202242109

Sneader W. 2005. Drug discovery: a history. England: John Wiley & Sons. ISBN: 9780471899792

Tu Y. 2011. The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine. Nature Medicine 17: 1217-1220. DOI: https://doi.org/10.1038/nm.2471

Tucker MC, Aze T, Cadotte MW, Cantalapiedra JL, Chisholm C, Díaz S, Grenyer R, Huang D, Mazel F, Pearse WD, Pennell MW, Winter M, Mooers AO. 2019. Assessing the utility of conserving evolutionary history. Biological Reviews 94: 1740-1760. DOI: https://doi.org/10.1111/brv.12526

Unschuld PU. 1986. Medecine in China: A History of Pharmaceutics. California: University of California Press. ISBN: 0520050258

Valdivia-Correa B, Gómez-Gutiérrez C, Uribe M, Méndez-Sánchez N. 2016. Herbal Medicine in Mexico: A Cause of Hepatotoxicity. A Critical Review. International Journal of Molecular Sciences 17: 235. DOI: https://doi.org/10.3390/ijms17020235

Villanueva-Almanza L. 2021. Cómo una planta ahora olvidada de los bosques tropicales mexicanos revolucionó la vida de las mujeres. Botany One. https://botany.one/2021/03/como-una-planta-ahora-olvidada-de-los-bosques-tropicales-mexicanos-revoluciono-la-vida-de-las-mujeres/ (Accessed 10 Feb, 2023)

Watanabe KN, Teh GH. 2011. Wanted: bioprospecting consultants. Nature Biotechnology 29: 873-875. DOI: https://doi.org/10.1038/nbt.2001

Watkins R, Wu L, Zhang C, Davis RM, Xu B. 2015. Natural product-based nanomedicine: recent advances and issues. International journal of nanomedicine 10: 6055-6074. DOI: https://doi.org/10.2147/IJN.S92162

WHO [World Health Organization]. 2018. Annex 1. WHO guidelines on good herbal processing practices for herbal medicines. Technical Report Series. Geneva, Switzerland: WHO headquarters.

Zhu F, Qin C, Tao L, Liu X, Shi Z, Ma X, Jia J, Tan Y, Cui C, Lin J, Tan C. 2011. Clustered patterns of species origins of nature-derived drugs and clues for future bioprospecting. Proceedings of the National Academy of Sciences 108: 12943-12948. DOI: https://doi.org/10.1073/pnas.1107336108

Zuluaga G, Correa C. 2002. Medicinas tradicionales: Introducción al estudio de los sistemas tradicionales de salud y su relación con la medicina moderna. Bogotá, DC: Editorial Kimpres Ltda. ISBN: 9588077540.

How to Cite
Cupido, M., De-Nova, J. A., & Cilia-López, V. G. (2023). Evolutionary approaches in ethnobotany of medicinal plants and bioprospecting. Botanical Sciences, 102(1), 26-38. https://doi.org/10.17129/botsci.3325