Morphoanatomical and phylogenetic characterization of the ectomycorrhiza between Laccaria squarrosa with Pinus pseudostrobus and its relevance for reforestation programs

keywords: Ectomycorrhizal symbiosis, edible wild mushrooms, inoculants, Neotropics, pines

Abstract

Background: Pinus (Coniferophyta) and Laccaria (Basidiomycota) establish ectomycorrhizal symbioses in natural forests. However, their detailed morphoanatomical and phylogenetic characterization have received little attention. Accurate identification of native host symbionts is of paramount relevance to the production of mycorrhized seedlings for successful reforestation programs.

Questions/Objective: We aimed to determine if L. squarrosa is able to establish ectomycorrhizal symbiosis with gymnosperms, thereby widening its host range and highlighting its relevance as a potential inoculant for pine seedlings. Currently, L. squarrosa is only known from its type collection associated with the angiosperm Fagus grandifolia var. mexicana.

Studied species: The fungus L. squarrosa and Pinus pseudostrobus, a tree endemic to Mexico.  

Study site and dates: A Pinus-Quercus forest in Piedra Canteada, Nanacamilpa, Tlaxcala; 2018-2020.

Methods: L. squarrosa basidiomata were identified and ectomycorrhizal roots were collected and morphoanatomically characterized. For molecular identification, DNA was extracted, PCR was performed targeting the nuclear ribosomal internal transcribed spacer region (nucrDNA ITS) for the mycobiont identification and the chloroplastic single-locus trnL region for the phytobiont.

Results: In the phylogenetic analyses, our sequences from basidiomata and ectomycorrhizae clustered together with L.squarrosa with high values of supporting identity. Meanwhile, P. pseudostrobus was molecularly identified as the phytobiont.

Conclusions: This is one of the few worldwide characterizations of Laccaria ectomycorrhiza under field conditions and contributes to the understanding of the ecology, distribution, and economic relevance of the symbiotic association. Our data suggest that L. squarrosa has potential for use as a native inoculant for P. pseudostrobus tree production.

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Author Biographies

Mariana Herrera, Germplasm Bank of Wild Species in Southwestern China, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan

Postdoctoral Position

Fu-Qiang Yu, Germplasm Bank of Wild Species, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan

Associate Professor 

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David Ramos-Rendón, Investigador independiente, Xalapa, Veracruz

Researcher

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Magdalena Martínez-Reyes, Colegio de Postgraduados, Edafología, Campus Montecillo, Texcoco

Researcher

Faustino Hernández-Santiago, Colegio de Postgraduados, Edafología, Campus Montecillo, Texcoco

Associate Professor

Jesus Perez-Moreno, Colegio de Postgraduados, Edafología, Campus Montecillo, Texcoco

Profesor Investigador Titular

Microbiologia, Edafologia

Campus Montecillo

Colegio de Postgraduados

Morphoanatomical and phylogenetic characterization of the ectomycorrhiza between <em>Laccaria squarrosa</em> with <em>Pinus pseudostrobus</em> and its relevance for reforestation programs

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Published
2021-12-15
How to Cite
Herrera, M., Yu, F.-Q., Ramos-Rendón, D., Martínez-Reyes, M., Hernández-Santiago, F., Chater, C. C., & Perez-Moreno, J. (2021). Morphoanatomical and phylogenetic characterization of the ectomycorrhiza between Laccaria squarrosa with Pinus pseudostrobus and its relevance for reforestation programs. Botanical Sciences, 100(2), 397-411. https://doi.org/10.17129/botsci.2830
Section
STRUCTURAL BOTANY / BOTÁNICA ESTRUCTURAL