Seed integrity, effect of temperature and storage time on germination of Populus luziarum and P. primaveralepensis, endangered subtropical species from Mexico

  • César Jacobo-Pereira Doctorado en Ciencias en Biosistemática, Ecología y Manejo de Recursos Naturales y Agrícolas, Universidad de Guadalajara, Zapopan, Jalisco https://orcid.org/0000-0003-1454-575X
  • Miguel Ángel Muñiz-Castro Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco https://orcid.org/0000-0002-9127-057X
  • J. Antonio Vázquez-García Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, JaliscoInstituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco http://orcid.org/0000-0002-8393-5906
  • Joel Flores Instituto Potosino de Investigación Científica y Tecnológica A.C., División de Ciencias Ambientales, San Luis Potosí https://orcid.org/0000-0001-8856-6022
  • Alejandro Muñoz-Urias Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco https://orcid.org/0000-0001-8856-6022
  • Francisco Martín Huerta-Martínez Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco https://orcid.org/0000-0001-6923-3425
keywords: Salicaceae, seed physical integrity, seed storage conditions, subtropical endemic species, white poplars

Abstract

Background: Populus luziarum and P. primaveralepensis are endemic species of western Mexico; growing in riparian forests they are critically endangered. The best way to conserve their seeds is unknown, which could be limiting for their conservation.

Hypothesis: The germinability of both subtropical species is like that of boreal and template Salicaceae species that disperse seeds in spring and early summer, as they germinate quickly with high percentages, and rapidly lose their viability when stored at ambient temperature.

Studied species: Populus luziarum and P. primaveralepensis.

Study site and dates: Western Trans-Mexican Volcanic Belt. Jalisco, Mexico. October 2019.

Methods: The physical integrity of the seeds was assessed by X-ray imaging and compared with germinability. In addition, the effect of storage time (nine weeks) under two temperatures (4 and 21 °C) on the percentage and mean germination rate was evaluated.

Results: No significant differences were found between physical integrity and germination in freshly collected seeds for both species. Germination in the first 24 hrs was 91 and 95 % for Populus luziarum and P. primaveralepensis, respectively (week 0). Germination percentages were lower when stored at 21 °C, but P. primaveralepensis was decreased more slowly.

Conclusions:  Seeds of subtropical Populus respond similarly to those of species from temperate and boreal climates with early seed dispersal, a crucial condition for establishing ex situ reforestation and conservation programs.

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Author Biographies

César Jacobo-Pereira, Doctorado en Ciencias en Biosistemática, Ecología y Manejo de Recursos Naturales y Agrícolas, Universidad de Guadalajara, Zapopan, Jalisco

Student of BEMARENA of Universidad de Guadalajara

Miguel Ángel Muñiz-Castro, Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco

Department of Botany and Zoology

 

J. Antonio Vázquez-García, Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, JaliscoInstituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco

Department of Botany and Zoology

Alejandro Muñoz-Urias, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco

Ecology Department

Francisco Martín Huerta-Martínez, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco

Department of Ecology

Seed integrity, effect of temperature and storage time on germination of <em>Populus luziarum</em> and <em>P. primaveralepensis</em>, endangered subtropical species from Mexico

References

Abud HF, Cicero SM, Gomes-Junior FG. 2018. Radiographic images and relationship of the internal morphology and physiological potential of broccoli seeds. Acta Scientiarum Agronomy 40: 1-9. DOI: https://doi.org/10.4025/actasciagron.v40i1.34950

Baskin CC, Baskin JM. 2014. Seeds: Ecology, biogeography, and evolution of dormancy and germination. San Diego: Elsevier Sciences. ISBN 13: 978-0-12-416677-6

Bonner FT. 2008. Storage of seed. In: Bonner FT, Karrfalt RP, eds. The woody plant seed manual agriculture Handbook 727, Washington DC. U.S. Department of Agriculture, Forest Service pp. 85-96. https://www.fs.usda.gov/treesearch/pubs/32626 (accessed May 20, 2019)

Chilpa-Galván N, Márquez-Guzmán J, Zotz G, Echevarría-Machado I, Andrade JL, Espadas Manrique C, Reyes-García C. 2018. Seed traits favouring dispersal and establishment of six epiphytic Tillandsia (Bromeliaceae) species. Seed Science Research 4: 349-359. DOI: https://doi.org/10.1017/S0960258518000247

Crawley MJ. 2012. The R Book. UK: John Wiley & Sons. ISBN 978-0-470-97392-9

De Medeiros AD, Araújo JO, León MJZ, Da Silva LJ, Dias DCFS. 2018. Parameters based on X-ray images to assess the physical and physiological quality of Leucaena leucocephala seeds. Ciência e Agrotecnologia 42: 643-652. DOI: https://doi.org/10.1590/1413-70542018426023318

De Medeiros AD, Zavala-León MJ, Da Silva LJ, Oliveira AMS, Dias DCFS. 2020. Relationship between internal morphology and physiological quality of pepper seeds during fruit maturation and storage. Agronomy Journal 112: 25-35. DOI: https://doi.org/10.1002/agj2.20071

Dickmann DI, Kuzovkina J. 2014. Poplars and willows of the world, with emphasis on silviculturally important species. In: Isebrands JG, Richardson J, eds. Poplars and Willows: Trees for Society and the Environment. Rome: CAB International and FAO, pp. 88-91. DOI: https://doi.org/10.1079/9781780641089.0008

Eckenwalder JE. 1996. Systematics and evolution of Populus. In: Stettler RF, Bradshaw Jr. HD, Heilman PE, Hinckley TM, eds. Biology of Populus and its implications for management and conservation, Part I, Ottawa: NRC Research Press, pp.7-32. ISBN: 0-660-16506-6

Fechner GH, Burr KE, Myers JF. 1981. Effects of storage, temperature, and moisture stress on seed germination and early seedling development of trembling aspen. Canadian Journal of Forest Research 11: 718-722. DOI: https://doi.org/10.1139/x81-100

Gomes-Junior FG, Chiquito AA, Marcos-Filho J. 2013. Semi-automated assessment of the embryonic area of cucumber seeds and its relationship to germination and seedling length. Journal of Seed Science 35: 183-189. DOI: https://doi.org/10.1590/S2317-15372013000200007

Gomes-Junior FG, Yagushi JT, Belini UL, Cicero SM, Tomazello-Filho M. 2012. X-ray densitometry to assess internal seed morphology and quality. Seed Science and Technology 40: 102-107. DOI: https://doi.org/10.15258/sst.2012.40.1.11

González E, Comín FA, Muller E. 2010. Seed dispersal, germination and early seedling establishment of Populus alba L. under simulated water table declines in different substrates. Trees 24: 151-163. DOI: https://doi.org/10.1007/s00468-009-0388-y

Gordon JC. 2001. Poplars: trees of the people, trees of the future. The Forestry Chronicle 77: 217-219. DOI: https://doi.org/10.5558/tfc77217-2

Harper JL. 1977. Population Biology of Plants. London: Academic Press, ISBN: 9780123258502

Holdridge LR. 1967. Life zone ecology. San José: Tropical Science Center, ISBN 13: 9780719518867

ISTA [International Seed Testing Association]. 2020. Rules for Seed Testing. International Seed Testing Association.

IUCN. 2019. IUCN Red List Categories and Criteria: Version 3.1. Second edition. Gland, Switzerland and Cambridge, UK: IUCN, iv +32 pp. https://www.iucnredlist.org/resources/categories-and-criteria (accessed March 14, 2019).

Javorski M, Castan DOC, Silva SS, Gomes-Junior FG, Cicero SM. 2018. Image analysis to evaluate the physiological potential and morphology of pearl millet seeds. Journal of Seed Science 40: 127-134. DOI: https://doi.org/10.1590/2317-1545v40n2176904

Javorski M, Moure S. 2017. Utilização de raios x na avaliaçãoda morfologia interna de sementes de sorgo. Revista Brasileira de Milho e Sorgo 16: 310-318. DOI: https://doi.org/10.18512/1980-6477/rbms.v16n2p310-318

Karrenberg S, Edwards PJ, Kollmann J. 2002. The life history of Salicaceae living in the active zone of floodplains. Freshwater Biology 47: 733-748. DOI: https://doi.org/10.1046/j.1365-2427.2002.00894.x

Kim DH. 2018. Extending Populus seed longevity by controlling seed moisture content and temperature. Plos One 13: p.e0203080. DOI: https://doi.org/10.1371/journal.pone.0203080

Maroder HL, Prego IA, Facciuto GR, Malldonado SB, Maldonado SB. 2000. Storage behaviour of Salix alba and Salix matsudana seeds. Annals of Botany 86: 1017-1021. DOI: https://doi.org/10.1006/anbo.2000.1265

Martínez-González RE, González-Villarreal LM. 2005. Taxonomía y biogeografía del género Populus, (Salicaceae) en México. BSc Thesis. Universidad de Guadalajara.

Moss EH. 1938. Longevity of Seed and establishment of seedlings in species of Populus. Botanical Gazette 99: 529-542.

Muller C, Teissier du Cros E, Laroppe E, Duval H. 1982. Conservation pendant 5 ans de graines de peupliers noirs (Populus nigra L.). Annales des Sciences Forestieres 39: 179-185. DOI: https://doi.org/10.1051/forest:19820205

Parsons RF. 2012. Incidence and ecology of very fast germination. Seed Science Research 22: 161-167. DOI: https://doi.org/10.1017/S0960258512000037

Paw?owski TA, Klupczy?ska EA, Staszak AM, Suszka J. 2019. Proteomic analysis of black poplar (Populus nigra L.) seed storability. Annals of Forest Science 76: 104. DOI: https://doi.org/10.1007/s13595-019-0887-y

Pence VC. 1996. Germination, desiccation and cryopreservation of seed of Populus deltoides Barr. Seed Science and Technology 24: 151-157.

Popova EV, Kim DH, Han SH, Moltchanova E, Pritchard HW, Hong YP. 2013. Systematic overestimation of Salicaceae seed survival using radicle emergence in response to drying and storage: implications for ex situ seed banking. Acta Physiol Plant 35: 3015-3025. DOI: https://doi.org/10.1007/s11738-013-1334-6

Pritchard HW, Nadarajan J. 2008. Cryopreservation of Orthodox (Desiccation Tolerant) Seeds. In: Reed BM. eds. Plant Cryopreservation: A Practical Guide. New York: Springer, pp. 485-501. DOI: https://doi.org/10.1007/978-0-387-72276-4_19

R Core Team. 2018. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/

Ranal MA, Santana DG. 2006. How and why to measure the germination process? Brazilian Journal of Botany 29: 1-11. DOI: https://doi.org/10.1590/S0100-84042006000100002

Ranal MA, Santana DGD, Ferreira WR, Mendes-Rodrigues C. 2009. Calculating germination measurements and organizing spreadsheets. Brazilian Journal of Botany 32: 849-855. DOI: https://doi.org/10.1590/S0100-84042009000400022

Ruiz-Corral JA, Medina-García G, García-Romero GE. 2018. Agroclimatic information system for Mexico-Central America. Revista Mexicana de Ciencias Agrícolas 9: 1-10. DOI: https://doi.org/10.29312/remexca.v9i1.843

Scott S, Jones R, Williams W. 1984. Review of data analysis methods for seed germination. Crop Science 24: 1192-1199. DOI: https://doi.org/10.2135/cropsci1984.0011183X002400060043x

SEMARNAP. 2000. Programa de Manejo Área de Protección de Flora y Fauna La Primavera, México. México, DF: Secretaría del Medio Ambiente, Recursos Naturales y Pesca.

Siegel RS, Brock JH. 1990. Germination requirements of key southwestern woody riparian species. Desert Plants 10: 3-8.

Suszka J, Plitta BP, Michalak M, Bujarska-Borkowska B, Tylkowski T, Chmielarz P. 2014. Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm. Annals of Forest Science 71: 543-549. DOI: https://doi.org/10.1007/s13595-014-0368-2

Tauer CG. 1979. Seed tree, vacuum, and temperature effects on eastern cottonwood seed viability during extended storage. Forest Science 25: 112-114. DOI: https://doi.org/10.1093/forestscience/25.1.112

Vandelook F, Janssens SB, Probert RJ. 2012. Relative embryo length as an adaptation to habitat and life cycle in Apiaceae. New Phytologist 195: 479-487. DOI: https://doi.org/10.1111/j.1469-8137.2012.04172.x

Vázquez-García JA, Cuevas-Guzmán R. 1989. Una nueva especie tropical de Populus (Salicaceae) de la Sierra de Manantlán, Jalisco, México. Acta Botánica Mexicana 8: 39-45. DOI: https://doi.org/10.21829/abm8.1989.586

Vázquez-García JA, Muñiz-Castro MA, Martínez-González RE, Nieves-Hernández G, Pulido-Ávila MG, Hernández-Vera G, Zuno-Delgadillo O. 2019. Populus primaveralepensis sp. nov. (Salicaceae, Malpighiales), a new species of white poplar from the Bosque La Primavera Biosphere Reserve in western Mexico. European Journal of Taxonomy 498: 1-16. DOI: https://doi.org/10.5852/ejt.2019.498

Vázquez-García JA, Muñiz-Castro MA, Padilla-Lepe J, Pulido MG, Nieves G, Martínez RE. 2017. Populus luzae (Salicaceae), a new species of white poplar endemic to the western Transmexican Volcanic Belt, in Zapopan, Jalisco, Mexico. Phytotaxa 328: 243-256. DOI: https://doi.org/10.11646/phytotaxa.328.3.3

Vázquez-Yanes C, Orozco-Segovia A. 1993. Patterns of seed longevity and germination in the tropical rainforest. Annual Review of Ecology and Systematics 24: 69-87. DOI: https://doi.org/10.1146/annurev.es.24.110193.000441

Venable DL, Brown JS. 1988. The selective interaction of dispersal, dormancy, and seed size as adaptation for reducing risk in variable environments. The American Naturalist 131: 360-384. DOI: https://doi.org/10.1086/284795

Wyckoff G, Zasada JC. Populus L. 2008. In: Bonner FT, Karrfalt RP, eds. The Woody Plant Seed Manual Agriculture Handbook 727, Washington DC. U.S; Department of Agriculture, Forest Service pp. 856-871. https://www.fs.usda.gov/treesearch/pubs/32626 (accessed May 18, 2019).

Zasada JC, Densmore RC. 1980. Alaskan willow and balsam poplar seed viability after 3 years storage. Tree Planters' Notes 31: 9-10. https://rngr.net/publications/tpn/31-2/31_2_9_10.pdf

Zasada JC, Viereck LA. 1975. The effect of temperature and stratification on germination in selected members of the Salicaceae in interior Alaska. Canadian Journal of Forest Research 5: 333-337. DOI: https://doi.org/10.1139/x75-045

Published
2021-11-10
How to Cite
Jacobo-Pereira, C., Muñiz-Castro, M. Ángel, Vázquez-García, J. A., Flores Rivas, J. D., Muñoz-Urias, A., & Huerta-Martínez, F. M. (2021). Seed integrity, effect of temperature and storage time on germination of Populus luziarum and P. primaveralepensis, endangered subtropical species from Mexico. Botanical Sciences, 100(1), 192-203. https://doi.org/10.17129/botsci.2810
Section
PHYSIOLOGY / FISIOLOGÍA