Seed longevity, viability and germination of four weed-ruderal Asteraceae species of ethnobotanic value

keywords: dormancy, ex situ conservation, mean germination time, seed bank, weeds

Abstract

Background: A high proportion of Asteraceae species are considered weeds, some of them have recognizable biological properties. To design production protocols and ex situ seed conservation programs is necessary to determine the effect of storage temperature on seed viability and longevity. It is known that cold temperatures maintain seed viability and thus prolong seed longevity.

Hypotheses: The seeds of Aldama dentata, Verbesina virgata, Stevia origanoides and Roldana barba-johannis stored at low temperature (5 ºC) will have greater longevity than when stored at room temperature (16.45 ± 1.94 oC) and will decrease their viability as they age.

Study site and dates: Seeds of the four Asteraceae were collected (November-December 2015) in The National Park “El Tepozteco”, Morelos, Mexico and in the “Chamilpa” campus of the Universidad Autónoma del Estado de Morelos.

Methods: The monthly germination experiments (11) were implemented with seeds stored under both temperatures, germination (%) and mean germination time were registered to estimate seed longevity. The seed viability stored at room temperature was evaluated with the tetrazolium test.

Results: Germination and viability decreased with seed age. Along the experiment, seed germination was similar at both storage temperatures. A. dentata and S. origanoides showed the highest germination but A. dentata alone reached the major seed viability. The oldest seeds germinated faster.

Conclusions: Seed longevity of all the species exceeded one year. For propagation purposes, it is recommended to use seeds between 8 and 9 months of age, when their germination and viability are the highest.

Downloads

Download data is not yet available.

Author Biography

Susana Valencia-Díaz, Centro de Investigación en Biotecnología (CEIB), UAEM. Cuernavaca, Morelos

Centro de Investigación en Biodiversidad y Conservación. Universidad Autónoma del Estado de Morelos

Seed longevity, viability and germination of four weed-ruderal Asteraceae species of ethnobotanic value

References

Baskin CC, Baskin JM. 1998. Seeds. Ecology, Biogeography and Evolution of Dormancy and Germination. San Diego California, U.S.A. Academic Press.
Benvenuti S, Cioni PL, Flamini G, Pardossi A. 2017. Weeds for weed control: Asteraceae essential oils as natural herbicides. Weed Research 57: 342–353. doi: 10.1111/wre.12266.
Bertolosi BA, Santos de Oliveira T, Apezzato-da-Glória B, Coehho NADL. 2015. Seed germination of Brazilian Aldama species (Asteraceae). Journal of Seed Science 37: 185–191. doi.org/10.1590/2317-1545v37n3146138.
Bretz F, Hothorn T, Westfall P. 2011. Multiple comparisons using R. New York. CRC Press.
Brunick R. 2007. Seed dormancy in domesticated and wild sunflowers (Helianthus annuus L.): types, longevity and QTL discovery. PhD Dissertation, Oregon State University, USA.
Buiting J, Leprince O. 2018. Letter to the twenty first century botanist second series: What is a seed?-2. Regulation of desiccation tolerance and longevity in developing seeds: two faces of the same coin. Botany letters 165: 181–185. doi/full/10.1080/23818107.2018.1476177.
Céspedes CL, Torres P, Mar?n JC, Arciniegas A, de Vivar AR, Pérez-Castorena AL, Aranda E. 2004. Insect growth inhibition by tocotrienols and hydroquinones from Roldana barba-johannis. Phytochemistry 65: 1963–1975. Doi:10.1016/j.phytochem.2004.03.037.
Chena-Becerra F, Palmeros-Sánchez B, Fernández MS, Lozada-García JA. 2014. Antimicrobial activity of nine medicinal plants from Veracruz, México. International Journal of Applied Biology and Pharmaceutical Technology 5: 113–119.
Cicevan R, Al Hassan M, Sestras AF, Prohens J, Vicente O, Sestras RE, Boscaiu MM. 2016. Screening for drought tolerance in cultivars of the ornamental genus Tagetes (Asteraceae). Peer J 4:e2133; DOI 10.7717/peerj.2133.
Crawley MJ. 1993. GLIM for ecologist. Oxford, UK. Blackwell.
Crawley MJ. 2013. The R book. Chichester, OK. Wiley.
Cruz-Fernández T, Alquicira-Arteaga ML, Flores-Palacios A. 2011. Is orchid species richness and abundance related to the conservation status of oak forest? Plant Ecology. 212:1091–1099. doi 10.1007/s11258-010-9889-4.
Dickie JB, Ellis RH, Kraak HL, Ryders K, Tompsett PB. 1990. Temperature and seed storage longevity. Annals of Botany 65: 197–204. doi.org/10.1093/oxfordjournals.aob.a087924.
Diniz S, Prado PI, Lewinsohn TM. 2010. Species richness in natural and disturbed habitats: Asteraceae and flower-head insects. Neotropical Entomology 39: 163–171. doi.org/10.1590/S1519-566X2010000200004.
Donohue K, Dorn L, Griffith C, Kim G, Aguilera A, Polissety CR, Schmitt J. 2005. The evolutionary ecology of seed germination of Arabidopsis thaliana: variable natural selection on germination timing. Evolution 59: 758–770. https://www.jstor.org/stable/3449024.
Fenner, Thompson K. 2005. The Ecology of Seeds. USA, Cambridge University Press.
Funk VA, Randall JB, Keeley S, Chan R, Watson L, Gemeinholzer B, Schilling E, Panero JL, Baldwin BG, García-Jacas N, Alfonso S, Jansen RK. 2005. Everywhere but Antarctica: Using a supertree to understand the diversity and distribution of the Compositae. Biologiske. Skrifter 55: 343–373.
García-Herrera P, Sánchez-Mata MC, Cámara M, Fernández-Ruíz V, Díez-Márques C, Molina M, Tardio J. 2014. Nutrient composition of six wild edible Mediterranean Asteraceae plants of dietary interest. Journal of Food Composition and Analysis 34: 163–170. doi.org/10.1016/j.jfca.2014.02.009.
Ghani A, Tehranifar A, Shooshtarian S, Boghrati M. 2011. Comparative study of ornamental potential of six Achillea species from Iran. South-Western Journal of Horticulture Biology and Environment 2: 139–155.
Gomes V, Fernandes GW. 2002. Germination of Baccharis dracunculifolia DC (Asteraceae). Acta Botanica Brasilica 16: 421–427. doi.org/10.1590/S0102-33062002000400005.
Guzmán-Pantoja LE, Lina-García LP, Bustos-Zagal G, Hernández-Velázquez VM. 2012. Current Status: Mexican Medicinal Plants with insecticidal potential. In: Rasooli I (Ed.), Bioactive Compounds in Phytomedicine. Croatia, InTechOpen.
Heinrich M, Robles M, West JE, Ortiz de Montellano BR, Rodríguez E. 1998. Ethnofarmacology of mexican Asteraceae (Compositae). Annual Review of Pharmacology and Toxicology 38: 539–565. doi:10.1146/annurev.pharmtox.38.1.539.
Hothorn T, Bretz F, Westfall P. 2008. Simultaneous Inference in General Parametric Models. Biometrical Journal 50: 346?363.
Kochanek J, Bucley YM, Probert RJ, Adkins SW, Steadman J. 2010. Pre-zygotic parental environment modulates seed longevity. Austral Ecology 35: 837–848. doi:10.1111/j.1442-9993.2010.02118.x.
Martínez M, Roma VA, Ortega A, Quintero MDL, García C, Fronezec FR. 1983 Eudesmane triols from Verbesina virgata. Phytochemistry 22: 979–982.
Medina-Medrano JR, Torres-Contreras JE, Valiente-Banuet JI, Mares-Quiñones MD, Vásquez-Sánchez M, Álvarez-Bernal D. 2018. Effect of solid liquid extraction solvent on the phenolic content and antioxidant activity on three species of Stevia leaves. Separation Science and Technology 54: doi.org/10.1080/01496395.2018.1546741.
Mora F, Alpan L, McCracken J, Nieto M. 2013. Chemical and biological aspects of the genus Verbesina. The Natural Products Journal 3:140–150. doi:10.2174/2210315511303020009.
Moreno ME. 1984. Análisis físico y biológico de semillas agrícolas. Instituto de Biología. Universidad Nacional Autónoma de México. D.F.
Ndihokubwayo N, Nguyen VT, Cheng D. 2016. Effects of origin, seasons and storage under different temperatures on germination of Senecio vulgaris. Peer J. doi: 10.7717/peerj.2346.
Nenaah GE, Ibrahim SIA, Al-Assiuty BA. 2015. Chemical composition, insecticidal activity and persistence of three Asteraceae essential oils and their nanoemulsions against Callosobruchus maculatus (F.). Journal of Stored Products Research 61: 9–16. doi.org/10.1016/j.jspr.2014.12.007.
Prebeg T, Bedran S, Žuti? I. 2019. The effect of mechanical stress on transplants of three ornamental Asteraceae species. Journal of Central European Agriculture 20: 365–375. doi:/10.5513/JCEA01/20.1.2063.
Probert RJ, Daws MI, Hay FR. 2009. Ecological correlates of ex situ seed longevity: a comparative study on 195 species. Annals of Botany 104: 57–69. doi:/10.1093/aob/mcp082.
Pyšek P. 1997. Compositae as invaders: Better than the others? Preslia 69: 9–22.
Rejmáneck M. 1996. A theory of seed plant invasiveness: the first sketch. Biological Conservation. 78: 171–181. doi.org/10.1016/0006-3207(96)00026-2.
Ranal MA, García de Santana D. 2006. How and why to measure the germination process. Revista Brasileira de Botanica 1: 1–11. doi.org/10.1590/S0100-84042006000100002.
Redwood ME, Mallack GR, Huebner CD. 2016. Seed longevity and dormancy state in a disturbance-dependent forest herb Ageratina altissima. Seed Science Research 26: 148–152. doi.org/10.1017/S0960258516000052.
Rodríguez MV, Bodrone MP, Castellari MP, Batlla D. 2018. Effect of storage temperature on dormancy release of sunflower (Helianthus annuus) achenes. Seed Science Research. 21: 101–111. doi.org/10.1017/S0960258518000065.
Rodríguez-Arévalo I, Mattana E, García L, Liu U, Pritchard HW, Ulian T. 2017. Conserving seeds of useful wild plants in Mexico: main issues and recommendations. Genetic Resources and Crop Evolution. 64: 1141–1190. doi:10.1007/s10722-016-0427-7.
Sakai AK, Allendorf FW, Holt JS, Lodge DM, Molofsky J, With KA, Baughman S, Cabin RJ, Cohen JE, Ellstrand NC, McCauley DE, O´Neil P, Parker IM, Thompson JN, Weller SG. 2001. The population biology of invasive species. Annual Review of Ecology and Systematics 32: 305–332.
Sano N, Rajjou L, North HM, Debeaujon I, Marion-Poll A, Seo M. 2016. Staying alive: Molecular aspects of seed longevity. Plant Cell and Physiology 57: 660–674. doi:10.1093/pcp/pcv186.
Solalinde-Vargas D. 2014. Área de actividad, uso de hábitat y conducta territorial de Bassariscus astutus en una zona periurbana. Tesis de Maestría en Biología Integrativa de la Biodiversidad y Conservación. Universidad Autónoma del Estado de Morelos. México
Stepp JR, Moerman DE. 2001. The importance of weeds in ethnopharmacology. Journal of Ethnopharmacology 75: 19–23. doi.org/10.1016/S0378-8741(00)00385-8.
Suárez-Mota, Villaseñor JL, Ramírez-Aguirre MB. 2018. Sitios prioritarios para la conservación de la riqueza florística y el endemismo de la Sierra Norte de Oaxaca, México. Acta Botánica Mexicana. 124: 1–28. doi.org/10.21829/abm124.2018.1296.
Tamado T, Schütz W, Milberg P. 2002. Germination ecology of the weed Parthenium hysterophorus in eastern Ethiopia. Annals of Applied Biology 140: 203–270. doi:10.1111/j.1744-7348.2002.tb00180.x.
Tielbörger K. Prasse R. 2009. Do seeds sense each other? Testing for density-dependent germination in desert perennial plants. Oikos 118: 792–800. doi: 10.1111/j.1600-0706.2008.17175.x.
Thompson K, Baker JP, Bekker RM. 1997. The soil seed banks of North West Europe: Methodology, density and longevity. Cambridge, Cambridge University Press.
Uçar E, Özyi?it Y, Turgut K. 2016. The effects of light and temperature on germination of Stevia (Stevia rebaudiana Bert.) seeds. Turkish Journal of Agriculture Research 3: 37–40. doi: 10.19159/tutad.76528.
Valencia-Díaz S, Montaña C. 2003. Effects of seed age, germination substrate, gibberelic acid, light and temperature on seed germination in Flourensia cernua (Asteraceae), a Chihuahuan Desert shurb. The Southwestern Naturalist 48: 1–13.
Van Etten ML, Conner JK, Chang SM, Baucom RS. 2017. Not all weeds are created equal: A data base approach uncovers differences in the sexual system of nature and introduced weeds. Ecology and Evolution 7: 2636–2642. doi:10.1002/ece3.2820.
Villaseñor JL, Ortiz E, Hinojosa-Espinosa O, Segura-Hernández G. 2011. Especies de la familia Asteraceae exóticas a la flora de México. UNAM, México.
Visscher AM, Seal CE, Newton RJ, Latorre FA, Pritchard HW. 2016. Dry seeds and environmental extremes: consequences for seed life span and germination. Functional Plant Biology 43: 656–668. doi.org/10.1071/FP15275.
Xuan T, Minh TD, Trubg KH, Khanhi TD. 2016. Allelopathic of sweet potato varieties to control weeds: Imperata cylindrica, Bidens pilosa and Ageratum conyzoides. Allelopathy Journal 38: 41–54.
Yuan X, Wen B. 2016. Seed germination response to light, temperature and water stress in three invasive Asteraceae weeds from Xishuangbanna, SW China. PLoS ONE 13(1):e0191710. https://doi.org/10.1371/journal.pone.0191710.
Published
2021-02-14
How to Cite
Jiménez-Vázquez, A. M., Flores-Palacios, A., Flores-Morales, A., Perea-Arango, I., Gutiérrez, M. del C., Arellano-García, J. de J., & Valencia-Díaz, S. (2021). Seed longevity, viability and germination of four weed-ruderal Asteraceae species of ethnobotanic value. Botanical Sciences, 99(2), 279-290. https://doi.org/10.17129/botsci.2743
Section
ECOLOGY / ECOLOGÍA