Phenolic compounds and antioxidant activity of methanolic extracts from leaves and flowers of chilcuague (Heliopsis longipes, Asteraceae)

keywords: Asteraceae, free radicals, foliage, phytochemicals, polyphenolic extracts


Background: Heliopsis longipes root is the widely used organ from the plant due to its culinary and medicinal importance. Nevertheless, during its collection the foliage is discarded. Therefore, it is required to perform studies about the phytochemical content with biological activity present in the different plant organs of the foliage, for a better exploitation of the plant.

Hypothesis: The concentration of phenolic compounds with antioxidant activity varies according to the type of plant organ and the geographic location of the plant collection.

Study species: Heliopsis longipes

Site and years of study: The H. longipes plants were collected in three locations (Conca, San Juan Buenaventura and Beltran) from Sierra Gorda of Mexico, between 2017 and 2018.

Methods: In this study, they were analyzed the methanolic extracts from H. longipes leaves and flowers, in order to determine the content of the phenolic compounds and scavenging of radicals activity (DPPH and ABTS•+).  Further characterization of the extracts was carried out by chromatography (UPLC).

Results: The total content of polyphenols, flavonoids and condensed tannins varied in depending on the type of plant organ and the origin site of H. longipes, being the leaves of Beltran those that represent the best source of phenolic compounds with scavenging activity of the radicals DPPH and ABTS•+. In the extracts, phenolic acids and flavonoids were identified.

Conclusions: The geographical climatic conditions influence the phenolic content and antioxidant activity of the methanolic extracts from leaves and flowers of H. longipes.


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Phenolic compounds and antioxidant activity of methanolic extracts from leaves and flowers of chilcuague (<em>Heliopsis longipes</em>, Asteraceae)


Andrade-Andrade G, Delgado-Alvarado A, Herrera-Cabrera BE, Arévalo-Galarza L, Caso-Barrera L. Variación de compuestos fenólicos totales, flavonoides y taninos en Vanilla planifolia Jacks. ex Andrews de la Huasteca Hidalguense, México. 2018. Agrociencia 52: 55-66.
Arituluk ZC, Cankaya IIT, Özkan AMG. 2016. Antioxidant Activity, Total Phenolic and Flavonoid Contens of Some Tanacetum L. (Asteraceae) Taxa Growing in Turkey. FABAD Journal of Pharmaceutical Sciences 41: 17-25.
Brahmi F, Mechri B, Dabbou S, Dhibi M, Hammami M. 2012. The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Industrial Crops and Products 38: 146-152. DOI:
Brillouet JM, Romieu C, Schoefs B, Solymosi K, Cheynier V, Fulcrand H, Verdeil J, Conéjéro G. 2013. The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Annals of Botany 112:1003-1014. DOI:
Caprioli G, Iannarelli R, Sagratini G, Vittori S, Zorzetto C, Sánchez-Mateo CC, Rabanal RM, Quassinti L, Bramucci M, Vitali LA, Petrelli D, Lupidi G, Venditti A, Maggi F. 2017. Phenolic acids, antioxidant and antiproliferative activities of Naviglio® extracts from Schizogyne sericea (Asteraceae). Natural Product Research 31: 515-522. DOI:
Cilia-López VG, Aguirre-Rivera JR, Espinosa-Reyes G, Flores-Cano JA, Reyes-Agüero JA, Juárez-Flores BI. 2014. Distribución de Heliopsis longipes (Heliantheae: Asteraceae), un recurso endémico del centro de México. Revista Chapingo Serie Ciencias Forestales y del Ambiente 20: 47-54. DOI:
Cilia-Lopez VG, Aguirre-Rivera JR, Reyes-Agüero JA, Juárez-Flores BI. 2008. Etnobotánica de Heliopsis longipes (Asteraceae: Heliantheae). Boletín de la Sociedad Botánica de México 83: 81-87. ISSN 0366-2128
Dias MI, Sousa MJ, Alves RC, Ferreira ICFR. 2016. Exploring plant tissue culture to improve the production of phenolic compounds: A review. Industrial Crops and Products 82: 9-22. DOI:
Edziri H, Jaziri R, Haddad O, Anthonissen R, Aouni M, Mastouri M, Verschaeve L. 2019. Phytochemical analysis, antioxidant, anticoagulant and in vitro toxicity and genotoxicity testing of methanolic and juice extracts of Beta vulgaris L. South African Journal of Botany 126: 170-175. DOI:
Escobedo-Martínez C, Guzmán-Gutiérrez SL, Hernández-Méndez MM, Cassani J, Trujillo-Valdivia A, Orozco-Castellanos LM, Enríquez RG. 2017. Heliopsis longipes: anti-arthritic activity evaluated in a Freund’s adjuvant-induced model in rodents. Revista Brasileira de Farmacognosia 27: 214-219. DOI:
Feregrino-Pérez AA, Torres-Pacheco I, Vargas-Hernández M, Munguía-Fragozo PV, Loarca-Piña GF, Mendoza-Díaz SO, Ocampo-Velázquez RV, Rico-García E, Guevara-González RG. 2011. Antioxidant and antimutagenic activities of Acacia pennatula pods. Journal of Scientific and Industrial Research 70: 859-864. ISSN: 0975-1084
Feregrino-Pérez, AA, Berumen, LC, García-Alcocer G, Guevara-González RG, Ramos-Gómez M, Reynoso-Camacho R, Acosta-Gallegos JA, Loarca-Piña G. 2008. Composition and chemopreventive effect of polysaccharides from common beans (Phaseolus vulgaris L.) on azoxymethane-induced colon cancer. Journal of Agricultural and Food Chemistry 56: 8737-8744. DOI:
García-Chávez A, Ramírez-Chávez E, Molina-Torres J. 2004. El género Heliopsis (Heliantheae; asteraceae) en México y las alcamidas presentes en sus raíces. Acta Botanica Mexicana 69: 115-131. ISSN: 0187-7151
Garcia-Mier L, Jimenez-Garcia SN, Guevara-González RG, Feregrino-Perez AA, Contreras-Medina LM, Torres-Pacheco I. 2015. Elicitor mixtures Compounds Significantly Increase Bioactive compounds, Antioxidant Activity, and Quality Parameters in Sweet Bell Pepper. Journal of Chemistry 2015: 1-8. DOI:
Gómez-Romero M, Segura-Carretero A, Fernández-Gutiérrez A. 2010. Metabolite profiling and quantification of phenolic compounds in methanol extracts of tomato fruit. Phytochemistry 71: 1848-1864. DOI:
González-Aguilar GA, González-Córdova AF, Vallejo-Cordoba B, Álvarez-Parrilla E, García HS. 2014. Los Alimentos Funcionales: Un nuevo reto para la industria de alimentos. México, D.F. AGT Editor, S.A. ISBN: 978-607-7551-37-9
Hernández I, Márquez L, Martínez I, Dieguez R, Delporte C, Prieto S, Molina-Torres J, Garrido G. 2009. Anti-inflammatory effects of ethanolic extract and alkamides-derived from Heliopsis longipes roots. Journal of Ethnopharmacology 124: 649–652. DOI:
Hossain ML, Monjur-Al-Hossain ASM, Sadhu SK. 2016. HPLC Profiling and Evaluation of In-vitro Antioxidant Activity of Cirsium arvense L. (Family: Asteraceae). Journal of Pharmacognosy and Phytochemistry 5: 272-277. ISSN: 2278-4136
Jamei R, Anvari D. 2018. Evaluation of Antioxidant Capacity and Phenolic Content in Ethanolic Extracts of Leaves and Flowers of Some Asteraceae Species. Recent Patents on Food, Nutrition & Agriculture 9: 42-49. DOI:
Jovanovi? AA, ?or?evi? VB, Zduni? GM, Pljevljakuši? DS, Šavikin KP, Go?evac DM, Bugarski BM. 2017. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. Separation and Purification Technology 179: 369-380. DOI:
Li FR, Liu LL, Liu JL, Yang K. 2019. Abiotic and biotic controls on dynamics of labile phosphorus fractions in calcareous soils under agricultural cultivation. Science of The Total Environment 681: 163-174. DOI:
Li Y, Kong D, Fu Y, Sussman MR, Wu H. (2020). The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiology and Biochemistry 148: 80-89. DOI:
Lin HY, Chang TC, Chang ST. 2018. A review of antioxidant and pharmacological properties of phenolic compounds in Acacia confusa. Journal of Traditional and Complementary Medicine 8: 443-450. DOI:
Manach C, Scalbert A, Morand C, Rémés C, Jiménez L. 2004. Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition 79: 727-747. DOI:
Mayouf N, Charef N, Saoudi S, Baghiani A, Khennouf S, Arrar L. 2019. Antioxidant and anti-inflammatory effect of Asphodelus microcarpus methanolic extracts. Journal of Ethnopharmacology 239: 111914. DOI:
Norma Oficial Mexicana NOM-021-RECNAT-2000. Especificaciones de fertilidad, salinidad y clasificación de suelos. Estudios, muestreo y análisis. Diario Oficial de la Federación. 2da Sección, 31 de diciembre de 2002.
Parola-Contreras I, Tovar-Perez EG, Rojas-Molina A, Luna-Vazquez FJ, Torres-Pacheco I, Ocampo-Velazquez RV, Guevara-González RG. 2020. Changes in affinin contents in Heliopsis longipes (chilcuague) after a controlled elicitation strategy under greenhouse conditions. Industrial Crops and Products 148: 112314. DOI:
Pretti IR, da Luz AC, Jamal CM, Batitucci MCP. 2018. Variation of biochemical and antioxidant activity with respect to the phenological stage of Tithonia diversifolia Hemsl. (Asteraceae) populations. Industrial Crops & Products 121: 241-249. DOI:
Quiroz-Sodi M, Mendoza-Díaz S, Hernández-Sandoval L, Carrillo-Ángeles I. 2018. Characterization of the secondary metabolites in the seeds of nine native bean varieties (Phaseolus vulgaris and P. coccineus) from Querétaro, Mexico. Botanical Sciences 96: 650-661. DOI:
Rodríguez-Méndez AJ, Carmen-Sandoval W, Lomas-Soria C, Guevara-González RG, Reynoso-Camacho R, Villagran-Herrera ME, Salazar-Olivo L, Torres-Pacheco I, Feregrino-Pérez AA. 2018. Timbe (Acaciella angustissima) Pods Extracts Reduce the Levels of Glucose, Insulin and Improved Physiological Parameters, Hypolipidemic Effect, Oxidative Stress and Renal Damage in Streptozotocin-Induced Diabetic Rats. Molecules 23: 2812. DOI:
Sampaio BL, Bara MTF, Ferri PH, da Costa Santos S, de Paula JR. 2011. Influence of environmental factors on the concentration of phenolic compounds in leaves of Lafoensia pacari. Brazilian Journal of Pharmacognosy 21: 1127-1137. DOI:
Sarmadi BH, Ismail A, Hamid M. 2011. Antioxidant and angiotensin converting enzyme (ACE) inhibitory activities of cocoa (Theobroma cacao L.) autolysates. Food Research International 44: 290-296. DOI:
Sindhi V, Gupta V, Sharma, K, Bhatnagar S, Kumari R, Dhaka N. 2013. Potential applications of antioxidants-A review. Journal of Pharmacy Research 7: 828-835. DOI:
Song B, Liang H, Sun R, Peng P, Jiang Y, She D. 2020. Hydrogel synthesis based on lignin/sodium alginate and application in agriculture. International Journal of Biological Macromolecules 144: 219-230. DOI:
Souyoul SA, Saussy KP, Lupo MP. 2018. Nutraceuticals: A Review. Dermatology and Therapy 8: 5-16. DOI:
Tapia RF, Patricia M, Sánchez O, Pedro J, Margez F, Martínez R, Alvarez E. 2019. Efecto de la salinidad y nitrógeno inorgánico del suelo en los compuestos fenólicos y capacidad antioxidante de Lycium berlandieri. Terra Latinoamericana 37: 81-90. DOI:
Toham H, Gulcin I, Bursal E, Goren A, Alwasel SH, Koksal I. 2017. Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS/MS. Food Measure 11: 556-566. DOI:
Vázquez-Hernández MC, Parola-Contreras I, Montoya-Gómez LM, Torres-Pacheco I, Schwarz D, Guevara-González RG. 2019. Eustressors: Chemical and physical stress factors used to enhance vegetables production. Scientia Horticulturae 250: 223-229. DOI:
Vergara-Castañeda HA, Guevara-González RG, Ramos-Gómez M, Reynoso-Camacho R, Guzmán-Maldonado H, Feregrino-Pérez AA, Oomah BD, Loarca-Piña G. 2010. Non-digestible fraction of cooked bean (Phaseolus vulgaris L.) cultivar Bayo Madero suppresses colonic aberrant crypt foci in azoxymethane-induced rats. Food and Function 1: 294-300. DOI:
Vu HT, Scarlett CJ, Vuong, QV. 2018. Phenolic compounds within banana peel and their potential uses: A review. Journal of Functional Foods 40: 238-248. DOI:
Willig JB, Salomón JLdO, Vianna DRB, Moura S, Pilger DA, Buffon A, Konrath EL. 2019. Heliopsis longipes S.F. Blake (Asteraceae) extract causes cell cycle arrest and induces caspase dependent apoptosis against cancer cell lines. South African Journal of Botany 125: 251-260. DOI:
Zhao C, Zhang H, Song C, Zhu JK, Shabala S. 2020. Mechanisms of Plant Responses and Adaptation to Soil Salinity. The Innovation 1: 100017. DOI:
How to Cite
Parola-ContrerasI., Guevara-GonzálezR. G., Feregrino-PérezA. A., Reynoso-CamachoR., Pérez-RamírezI. F., Ocampo-VelázquezR. V., Rojas- MolinaA., Luna-VazquezF., & Tovar-PérezE. G. (2020). Phenolic compounds and antioxidant activity of methanolic extracts from leaves and flowers of chilcuague (Heliopsis longipes, Asteraceae). Botanical Sciences, 99(1), 149-160.