Experimental seed germination for ex situ conservation of Mexican Podostemaceae

Valeria Flores-Enríquez, Guillermo Castillo, Margarita Collazo-Ortega

Abstract


Background: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (e.g., ex situ seed banks) of Mexican podostemads.

Hypotheses: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.

Studied species: Marathrum foeniculaceum Humb. & Bonpl., Noveloa coulteriana (Tul.) C. Philbrick

Study site and years of study: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. 

Methods: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.

Results: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. N. coulteriana have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of N. coulteriana more than in M. foeniculaceum.

Conclusions: Both species can form ex situ seed banks up to 8 yr age in paper bags storage. N. coulteriana is more susceptible to variation in storage conditions compared to M. foeniculaceum. Collection site affect seed germination after storing.  Long-term conventional ex situ seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.


Keywords


Podostemaceae, seed germination, germplasm, seed bank, Marathrum foeniculaceum, Noveloa coulteriana

Full Text:

PDF

References


Baskin CC, Baskin JM. 2014. Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination. San Diego: Academic Press. ISBN: 978-0-12-416677-6

Bawa KS, Kress WJ, Nadkarni NM, Lele S, Raven PH, Janzen DH, Lugo AE, Ashton PS, Lovejoy TE. 2004. Tropical ecosystems into the 21st century. Science 306: 227-228. DOI: https://doi.org/10.1126/science.306.5694.227b

Berjak PN, Pammenter NW. 2010. Semillas Ortodoxas y Recalcitrantes. In: Vozzo JA, Ed. Manual de Semillas de Árboles Tropicales. IV. US. Agricultural Department. Forestal Service, pp. 143-155.

Bewley JD, Bradford K, Hilhorst H, Nonogaki H. 2013. Seeds: physiology of development, germination and dormancy.Germany: Springer-Verlag. ISBN: 978-1-4614-4693-4

Castillo G, Márquez-Guzmán J, Collazo-Ortega M. 2013. Seed germination and early development in seedlings of Noveloa coulteriana (Podostemaceae). Aquatic Botany 109: 25-30. DOI: https://doi.org/10.1016/j.aquabot.2013.03.007

Castillo G, Flores-Enríquez V, Márquez-Guzmán J, Núñez-Farfán J, Oyama K, Collazo-Ortega M. 2017. Coping with stressful environments: An experimental study of seed germination and seedling survival of Mexican riverweeds under natural conditions. Aquatic Botany 138:24-28. DOI: https://doi.org/10.1016/j.aquabot.2016.12.006

Cook C, Rutishauser R. 2007. Podostemaceae. In: Kubitzki K, ed. Flowering Plants· Eudicots. Berlin: Springer: 304-344. DOI: https://doi.org/10.1007/978-3-540-32219-1_40; Print ISBN 978-3-540-32214-6 Online ISBN 978-3-540-32219-1

Dudgeon D, Arthington AH, Gessner MO, Kawabata ZI, Knowler DJ. Lévêque C, Naiman RJ, Prieur-Richard AH, Soto D, Stiassny ML, Sullivan CA. 2006. Freshwater biodiversity: importance, threats, status and conservation challenges. Biological Reviews 81:163-182. DOI: https://doi.org/10.1017/S1464793105006950

Garcia-Posada JA, Muñoz- López MT. 2011, Plantas acuáticas del altiplano del oriente antioqueño. Colombia. (accessed May 9, 2018).

Hong T, Ellis RH. 1996. A Protocol to Determine Seed Storage Behavior. Rome. IPGRI ISBN: 92-9043-279-9

Kami C, Lorrain S, Hornitschek P, Fankhauser C. 2010. Light-Regulated Plant Growth and Development. In: Timmermans MCP Ed. Current Topics in Developmental Biology vol 91 Academic Press, pp. 29-66. DOI: https://doi.org/10.1016/S0070-2153(10)91002-8

Katayama N, Kato M, Imaichi R. 2016. Habitat specificity enhances genetic differentiation in two species of aquatic Podostemaceae in Japan. American Journal of Botany 103:317-324. DOI: https://doi.org/10.3732/ajb.1500385

Luna R, Guzmán-Merodio D, Núñez-Farfán, Philbrick C, Collazo-Ortega M, Márquez-Guzmán J. 2012. Cross compatibility between Marathrum rubrum and Marathrum schiedeanum (Podostemaceae), two closely related species of the Pacific Mexican Coast. Aquatic botany 102: 1-7. DOI: https://doi.org/10.1016/j.aquabot.2012.02.001

Li DZ, Pritchard HW. 2009. The science and economics of ex situ plant conservation. Trends in Plant Science 14: 614-621. DOI: https://doi.org/10.1016/j.tplants.2009.09.005

Masahiro K, Lansdown RV. 2012. The Podostemaceae - riverweeds. In: Allen D, Smith K, Darwall W, comps. The Status and Distribution of Freshwater Biodiversity in Indo-Burma. Switzerland: IUCN Cambridge and Gland Switzerland, pp. 117-133. ISBN: 978-2-8317-1424-0

Mittermeier RA, Myers N, Thomsen JB, Da Fonseca G, Olivieri S. 1998. Biodiversity hotspots and major tropical wilderness areas: approaches to setting conservation priorities. Conservation Biology 12: 516-520. DOI: https://doi.org/10.1046/j.1523-1739.1998.012003516.x

Novelo A, Philbrick CT. 1997. Taxonomy of Mexican Podostemaceae. Aquatic Botany 57: 275-303. DOI: https://doi.org/10.1016/S0304-3770(96)01122-9

Novelo A, Philbrick C, Crow G. 2009. Podostemaceae. Flora Mesoamericana 3: 1-7.

Ojeda AO. 2011. Alteración de los regímenes de caudales de los ríos. Recuperación de riberas. España: Fundación Banco Santander. ISBN: 978-84-92543-23-6

Orozco-Segovia A, Sánchez-Coronado E. 2013. Germinación. In: Márquez-Guzmán J, Collazo-Ortega M, Martínez-Gordillo M, Orozco-Segovia A, Vázquez-Santana S, eds. Biología de Angiospermas, Ciudad de México: Las Prensas de Ciencias, pp. 212-240. ISBN: 978-607-02-2705-9

Pérez AG, Cuevas ML, Cotler H, González DI, Tharme R. 2010. Evaluación del grado de alteración ecohidrológica de los ríos y corrientes superficiales de México. Investigación Ambiental Ciencia y Política Pública 2: 25-46.

Philbrick CT. 1984. Aspects of floral biology, breeding system and seed and seedling biology in Podostemum ceratophyllum (Podostemaceae). Systematic Botany 9: 166-174. DOI: 10.2307/2418821

Philbrick CT, Bove CP, Stevens HI. 2010. Endemism in neotropical Podostemaceae. Annals of the Missouri Botanical Garden 97: 425-456. DOI: https://doi.org/10.3417/2008087

Philbrick CT, Novelo A. 1994. Seed germination of mexican podostemaceae. Aquatic Botany 48:145-151. DOI: http://dx.doi.org/10.1016/0304-3770(94)90081-7

Philbrick CT, Novelo A. 1995. New World Podostemaceae: ecological and evolutionary enigmas. Brittonia 47: 210-222. DOI: https://doi.org/10.2307/2806959

Philbrick CT, Novelo A.1997. Ovule number, seed number and seed size in Mexican and North American species of Podostemaceae. Aquatic Botany 57: 183-200. DOI: https://doi.org/10.1016/S0304-3770(96)01121-7

Quiroz FA, Novelo A, Philbrick CT. 1997. Water chemistry and the distribution of Mexican Podostemaceae: a preliminary evaluation. Aquatic botany 57: 201-212. DOI: https://doi.org/10.1016/S0304-3770(96)01118-7

R Core Team. 2011. R, a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. (accessed November 23, 2015)

Reyes-Ortega I, Sánchez-Coronado ME, Orozco-Segovia A. 2009. Seed germination in Marathrum schiedeanum and M. rubrum (Podostemaceae). Aquatic Botany 90: 13-17. DOI: http://dx.doi.org/10.1016/j.aquabot.2008.04.011

Sala OE, Chapin FS, Armesto JJ, Berlow E, Bloomfield J, Dirzo R, Huber-Sanwald E, Huenneke LF, Jackson RB, Kinzig A, Leemans, Lodge DM, Mooney HA, Oesterheld M, LeRoy Poof N, Sykes MT, Walker BH, Walker M, Wall DH. 2000. Global biodiversity scenarios for the year 2100. Science 287: 1770-1774. DOI: https://doi.org/10.1126/science.287.5459.1770

Sawada Y, Katsumata T, Kitamura J, Kawaide H, Nakajima M, Asami T, Nakaminami K, Kurahashi T, Mitsuhashi W, Inoue Y, Toyomasu, T. 2008. Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness. Journal of Experimental Botany 59: 3383-3393. DOI: https://doi.org/10.1093/jxb/ern192

SEMARNAT [Secretaría del Medio Ambiente y Recursos Naturales]. 2010. Norma Oficial Mexicana NOM-059-SEMARNAT-2010, Protección ambiental – Especies nativas de México de flora y fauna silvestres – Categorías de riesgo y especificaciones para su inclusión, exclusión o cambio – Lista de especies en riesgo. Diario Oficial de la Federación. 2da Sección, 30 de diciembre de 2010.

Sharrock SL. 2012. GSPC: Global Strategy for Plant Conservation: a Guide to the GSPC: All the Targets, Objectives and Facts. Richmond: Botanic Gardens Conservation International. (accessed February 22, 2016)

Tippery NP, Philbrick CT, Bove C, Les DH. 2011. Systematics and phylogeny of neotropical riverweeds (Podostemaceae: Podostemoideae). Systematic Botany 36: 105-118. DOI: https://doi.org/10.1600/036364411X553180

Vörösmarty CJ, McIntyre PB, Gessner MO, Dudgeon D, Prusevich A, Green P, Glidden S, Bunn SE, Sullivan CA, Liermann CR, Davies PM. 2010. Global threats to human water security and river biodiversity. Nature 467: 555-561. DOI: http://dx.doi.org/10.1038/nature09440

Waterworth WM, Bray CM, West CE. 2015. The importance of safeguarding genome integrity in germination and seed longevity. Journal of Experimental Botany, 66: 3549-3558. DOI: https://doi.org/10.1093/jxb/erv080

Wulff RD. 2017. Environmental maternal effects on seed quality and germination. In: Kigel J, ed. Seed Development and Germination. New York: Routledge, pp. 491-505. ISBN: 1351417312, 9781351417310




DOI: http://dx.doi.org/10.17129/botsci.2232

Article Metrics

Abstract Views.
Total number of Abstract Views for this article.
a description of the source 61
This journal








Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


 

Botanical Sciences is an international peer-reviewed journal that publishes scientific papers in plant sciences. The arguments, figures / schemes / photographs, quality and the general contents of this publication are full responsibility of the authors, and not commit the Editor- in-Chief or the Sociedad Botánica de México.

Botanical Sciences year 8, Vol. 97, No. 3, July-September 2019. Quarterly publication edited and published by Sociedad Botánica de México A.C. (www.socbot.mx). Editor in Chief Salvador Arias, Jardín Botánico, Instituto de Biología, 3er Circuito s/n, Ciudad Universitaria, Delegación Coyoacán, C.P. 04510. Reserves of Rights to the Exclusive Use No. 04-2017-040716054100-203, digital-ISSN 2007-4476, both granted by the Instituto Nacional del Derecho de Autor. Responsible for updating the page Pedro López, email: plopez@escire.mx, eScire. Last update July 18, 2019.

Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

  

 

website counter