Diversidad de la morfoanatomía foliar en Echeveria aff. gigantea (Crassulaceae)

Leaf morpho-anatomical diversity in Echeveria aff. gigantea (Crassulaceae)

Estela Sandoval-Zapotitla, Daniel M. Martínez-Quezada, Jerónimo Reyes-Santiago, María de los Ángeles Islas-Luna, Ulises Rosas

Abstract


Background. Distinguishing species and populations in Crassulaceae is challenging because of the morphological variability and inter-specific hybridization. Currently our understanding of the morphological and anatomical features in Echeveria is poor, and therefore it is difficult to delimit species, and morphotypes within the species. 

Question. Our objective was to describe the foliar anatomy and the shape of accessions in E. gigantea. We used E. gibbiflora, another species in the series as a comparison group. Comparing the two species allowed us to evaluate the relationship between anatomy and morphology in E. gigantea.

Methods. We performed a survey of anatomical features in seven accessions of E. gigantea, and one accession of E. gibbiflora. We obtained epidermal prints, leaf sections, performed geometric and morphometric analyses.

Results. We found that 65  % of the anatomical traits are heterogeneous among the taxonomic units. Our analysis showed that E. gibbiflora and some E. gigantea accessions share extensive anatomical similarities. There was variation within the E. gigantea, suggesting that at least one of the accessions is an independent taxonomic group. The traits with the largest contribution to variation between the groups were related to the epidermis, the hypodermis, the type of vascular bundles and the collenchyma associated to the vascular bundles. In addition we quantified the variation in leaf shape. Interestingly, we found correlations between the organ shape and anatomical traits.

Conclusion. These analyses provide information about traits towards the morpho-anatomical definition of E. gigantea variation and suggest developmental correlation yet to be explained.


Keywords


Developmental constraints; evolution; leaf anatomy; leaf shape; multivariate analysis; natural variation

References


Abdel-Raouf HS. 2012. Anatomical traits of some species of Kalanchoe (Crassulaceae) and their taxonomic value. Annals of Agricultural Sciences 57: 73-79. DOI: http://doi.org/10.1016/j.aoas.2012.03.002

Ardelean M, Stanescu I, Cachita-Cosma D. 2009. Sedum telephium L. spp. Maximum (L.) KROCK.- Histo-anatomical aspects on the vegetative organs. Scientific Annals of Alexandru Ioan Cuza University of Iasi. New Series 55: 75-80.

Arteaga MC, Bello-Bedoy R, de la Luz JLL, Delgadillo J, Domínguez R. 2015. Phenotypic variation of flowering and vegetative morphological traits along the distribution for the endemic species Yucca capensis (Agavaceae). Botanical Sciences 93: 765-770. DOI: http://doi.org/10.17129/botsci.214

Caballero-Ruano A, Jiménez-Parrondo MS. 1978. A contribution to the leaf anatomy studies of canarian crasuláceas. Vieraea 7: 115-132.

Castellón-Huerta BR. 2006. Cuthá: el cerro de la máscara: arqueología y etnicidad en el sur de Puebla. Mexico City: INAH. ISBN: 9789680301607

Chernetskyy M, Weryszko-Chmielewska E. 2008. Structure of Kalanchoë pumila Bak. leaves (Crassulaceae DC.). Acta Agrobotanica 61: 11-24. DOI: http://doi.org/10.5586/aa.2008.029

Chernetskyy MA. 2012. The role of morpho-anatomical traits of the leaves in the taxonomy of Kalanchoideae Berg. subfamily (Crassulaceae DC.). Modern Phytomorphology 1: 15-18. DOI: http://doi.org/10.5281/zenodo.162711

Costica M, Costica N, Toma O. 2007. Phytocoenological, histo-anatomical and biochemical aspects in Rhodiola rosea L. species from Romania. Analele Ştiinţifice ale Universităţii Alexandru Ioan Cuza, Secţiunea Genetică şi Biologie Moleculară, TOM VIII, 119-121.

Czernecki M. 2006. Mikromorfologia epidermy liści wybranych gatunków Kalanchoë Adans. /Micromorphology of leaf’s epidermis of some species of Kalanchoë Adans. Rocznik EKPiUU 3: 371-380.

Dickison WC. 2000. Integrative Plant Anatomy. Burlington: Academic Press. ISBN: 978-0-12-215170-5

Fahn A. 1969. Plant anatomy. Cornell University, USA: Pergamon Press.

García M, Lapp M. 2005. Anatomía foliar en especímenes de Oyedaea verbesinoides DC. (Asteraceae) creciendo en tres ambientes distintos. Ernstia 15: 129-143.

Hyakutake S, Souza AG. 1972. Contribuicao para o estudo morfologico e anatomico da Kalanchoe brasiliensis Cambessedes (Crassulaceae). Revista de Farmacia E Bioquimica Da Universidade De Sao Paolo 10: 217-237.

Inamdar JA, Patel RC. 1970. Structure and Development of Stornata in Vegetative and Floral Organs of Three Species of Kalanchoe. Annals of Botany, 34: 965-974.

Jones LA. 2011. Anatomical adaptations of four Crassula species to water availability. Bioscience Horizons: The International Journal of Student Research 4: 13-22. DOI: http://doi.org/10.1093/biohorizons/hzr002

Karwowska K, Brzezicka E, Kozieradzka-Kiszkurno M, Chernetskyy M. 2015. Anatomical structure of the leaves of Crassula cordata (Crassulaceae). Modern Phytomorphology 8: 53-54. DOI: http://doi.org/10.5281/zenodo.159830

Langlade NB, Feng X, Dransfield T, Copsey L, Hanna AI, Thebaud C, Bangham A, Hudson A, Coen E. 2005. Evolution through genetically controlled allometry space. Proceedings of the National Academy of Sciences of the United States of America 102: 10221-10226. DOI: http://doi.org/10.1073/pnas.0504210102

Moreira NS, Nascimento LBS, Leal-Costa MV, Tavares ES. 2012. Comparative anatomy of leaves of Kalanchoe pinnata and K. crenata in sun and shade conditions, as a support for their identification. Revista Brasileira de Farmacognosi 22: 929-936. DOI: http://doi.org/10.1590/S0102-695X2012005000056

Moteetee A, Nagendran CR. 1997. Comparative anatomical studies in five southern African species of Crassula: II. Structure of the leaf and the occurrence of transfer cells. South African Journal of Botany 63: 95-99. DOI: http://doi.org/10.1016/S0254-6299(15)30709-2

Mott KA, Gibson AC, O'Leary JW. 1982. The adaptive significance of amphistomatic leaves. Plant, Cell and Environment 5: 455-460. DOI: http://doi.org/10.1111/1365-3040.ep11611750

Mott KA, Michaelson O. 1991. Amphistomy as an adaptation to high light intensity in Ambrosia cordifolia (Compositae). American Journal of Botany 78: 76-79. DOI: http://doi.org/10.2307/2445230

Parkhurst DF. 1978. The adaptive significance of stomatal occurrence on one or both surfaces of leaves. Journal of Ecology 66: 367-383. DOI: http://doi.org/10.2307/2259142

Pilbeam J. 2008. The Genus Echeveria. Hornchurch, UK: British Cactus and Succulent Society. ISBN-13: 978-0902099807

Reyes-Santiago J, Brachet-Ize C, Gonzalez-Zorzano O, Islas-Luna A, Lopez-Chávez L. 2015. Four New Taxa of the Genus Echeveria from the State of Oaxaca, Mexico. Haseltonia 21: 80-91. DOI: http://doi.org/10.2985/026.021.0112

Reyes-Santiago JP, Islas-Luna MA, González-Zorzano O, Carrillo-Reyes P, Vergara-Silva F, Brachet-Ize C. 2011. Manual del perfil diagnóstico del género Echeveria en México. México: Universidad Autónoma Chapingo. ISBN: 978-607-12-0218-5

Rivas-Castro F. 2003. Cartografía antigua y sitios arqueológicos en la región de Reyes Metzontla, sureste de Puebla. Arqueologí, I: 142-155.

Rosas U, Barton NH, Copsey L, Barbier-de-Reuille P, Coen E. 2010. Cryptic Variation between Species and the Basis of Hybrid Performance. PLOS Biology 8: e1000429. DOI: http://doi.org/10.1371/journal.pbio.1000429

Rost TL. 1969. Vascular Pattern and Hydathodes in Leaves of Crassula argentea (Crassulaceae). Botanical Gazette 130: 267-270. DOI: http://doi.org/10.1086/336503

Rotondi A, Rossi F, Asunis C, Cesaraccio C. 2003. Leaf xeromorphic adaptations of some plants of a coastal Mediterranean macchia ecosystem. Journal of Mediterranean Ecology 4: 25-35.

Sandoval-Zapotitla E. 2005. Técnicas Aplicadas al Estudio de la Anatomía Vegetal. Mexico D.F.: Cuadernos del Instituto de Biología 38. Universidad Nacional Autónoma de México. ISBN: 9789703231317

Sandoval-Zapotitla E, García-Cruz J, Terrazas T, Villaseñor JL. 2010. Relaciones filogenéticas de la subtribu Oncidiinae (Orchidaceae) inferidas a partir de caracteres estructurales y secuencias de ADN (ITS y matK): un enfoque combinado. Revista Mexicana de Biodiversidad 81: 263-279.

Schlichting CD, Piggliucci M. 1998. Phenotypic Evolution: A Reaction Norm Perspective. Sinauer Associates, Sunderland. ISBN-13: 978-0878937998

Sharma GK, Dunn DB. 1968. Effect of Environment on the Cuticular Features in Kalanchoe fedschenkoi. Bulletin of the Torrey Botanical Club 95: 464-473. DOI: http://doi.org/10.2307/2483478

Stern FLSWL, Sweitzer EM, Phipps RE. 1970. Comparative anatomy and systematic of woody Saxifragaceae. Ribes. Botanical Journal of the Linnean Society 63: 215-237. DOI: http://doi.org/10.1111/j.1095-8339.1974.tb01744.x

Taiz L, Zeiger E. 2010. Plant Physiology. Sunderland: Sinauer Associates, Inc. ISBN-13: 978-0878938667

Tolken HR. 1977. A revision of the genus Crassula in southern Africa. University of Cape Town: The Bolus Herbarium.

Willmer C. 1983. Los estomas. Argentina: Librería Agropecuaria. ISBN-13: 978-9509350045

Willmer C, Fricker M. 1996. Stomata. London: Chapman and Hall. ISBN 0412574306, 9780412574306

Wu LH, Liu YJ, Zhou SB, Guo FG, Bi D, Guo XH, Baker AJM, Smith JAC, Luo YM. 2013. Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu (Crassulaceae): a new species from Zhejiang Province, China. Plant Systematics and Evolution, 299: 487-498. DOI: http://doi.org/10.1007/s00606-012-0738-x




DOI: http://dx.doi.org/10.17129/botsci.2028

Article Metrics

Abstract Views.
Total number of Abstract Views for this article.
a description of the source 420
This journal








Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


 

Botanical Sciences is an international peer-reviewed journal that publishes scientific papers in plant sciences. The arguments, figures / schemes / photographs, quality and the general contents of this publication are full responsibility of the authors, and not commit the Editor- in-Chief or the Sociedad Botánica de México.

Botanical Sciences year 8, Vol. 97, No. 3, July-September 2019. Quarterly publication edited and published by Sociedad Botánica de México A.C. (www.socbot.mx). Editor in Chief Salvador Arias, Jardín Botánico, Instituto de Biología, 3er Circuito s/n, Ciudad Universitaria, Delegación Coyoacán, C.P. 04510. Reserves of Rights to the Exclusive Use No. 04-2017-040716054100-203, digital-ISSN 2007-4476, both granted by the Instituto Nacional del Derecho de Autor. Responsible for updating the page Pedro López, email: plopez@escire.mx, eScire. Last update July 18, 2019.

Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

  

 

website counter